Typical Testing Data/Standard Curve (for reference only)
Rat Calcitonin Gene Related Peptide (CGRP) ELISA Kit | CGRP elisa kit
Rat Calcitonin Gene Related Peptide (CGRP) ELISA Kit
Reactivity
Rat
Synonyms
Calcitonin Gene Related Peptide (CGRP); Rat Calcitonin Gene Related Peptide (CGRP) ELISA Kit; CALCB; CGRP2; CALC2; CGRP-II; Calcitonin Gene Related Peptide 2; Calcitonin 2; Calcitonin Related Polypeptide Beta; CGRP elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of CGRP. No significant cross-reactivity or interference between CGRP and analogues was observed.
Sequence Length
134
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Type
Competitive inhibition
Detection Range
15.62-1000pg/mL
Sensitivity
4.86pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CGRP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CGRP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CGRP elisa kit
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of CGRP in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to CGRP has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled CGRP and unlabeled CGRP (Standards or samples) with the pre-coated antibody specific to CGRP. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of CGRP in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of CGRP in the sample.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to CGRP has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled CGRP and unlabeled CGRP (Standards or samples) with the pre-coated antibody specific to CGRP. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of CGRP in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of CGRP in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
14,965 Da
NCBI Official Full Name
calcitonin gene-related peptide 2
NCBI Official Synonym Full Names
calcitonin-related polypeptide, beta
NCBI Official Symbol
Calcb
NCBI Protein Information
calcitonin gene-related peptide 2
UniProt Protein Name
Calcitonin gene-related peptide 2
UniProt Gene Name
Calcb
UniProt Synonym Gene Names
CGRP-II
NCBI Description
may play a role in regulating transduction of sensory and motor information [RGD, Feb 2006]
Uniprot Description
CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role.
Research Articles on CGRP
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Product Notes
The Rat CGRP calcb (Catalog #AAA454057) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA454057 ELISA Kit recognizes Rat CGRP. It is sometimes possible for the material contained within the vial of "Calcitonin Gene Related Peptide (CGRP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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