Testing Data
(Rat spinal cord dorsal horn.)
Chicken anti-Rat Calcitonin Gene-Related Peptide Polyclonal Antibody | anti-CALCB antibody
Calcitonin Gene-Related Peptide (CGRP)
Gene Names
CALCB; CGRP
Reactivity
Rat
Applications
Immunohistochemistry
Purity
SerumSerum
Synonyms
Calcitonin Gene-Related Peptide; Polyclonal Antibody; Calcitonin Gene-Related Peptide (CGRP); Anti -Calcitonin Gene-Related Peptide (CGRP); anti-CALCB antibody
Host
Chicken
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgY
Specificity
Recognizes rat CGRP.
Purity/Purification
Serum
Serum
Serum
Form/Format
Supplied as a liquid, 0.05% sodium azide.
Applicable Applications for anti-CALCB antibody
Immunohistochemistry (IHC)
Application Notes
Suitable for use in Immunohistochemistry.
Dilution: Immunohistochemistry: 1:100-1:500. Used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode's solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at RT. C0115-01G was diluted with blocking buffer. Blocking buffer was removed from slides and they were then incubated for 18-24 hours at 4ºC with diluted C0115-01G. Slides were rinsed 3x and then incubated with secondary antibody for 1 hour at RT. Slides were again rinsed 3x and coverslipped. Staining was examined using fluorescence microscopy.
Dilution: Immunohistochemistry: 1:100-1:500. Used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode's solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at RT. C0115-01G was diluted with blocking buffer. Blocking buffer was removed from slides and they were then incubated for 18-24 hours at 4ºC with diluted C0115-01G. Slides were rinsed 3x and then incubated with secondary antibody for 1 hour at RT. Slides were again rinsed 3x and coverslipped. Staining was examined using fluorescence microscopy.
Immunogen
Synthetic peptide KDNFVPTNVGSEAF-NH2 corresponding to rat CGRP.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Testing Data
(Rat spinal cord dorsal horn.)
Testing Data
(Rat spinal cord dorsal horn.)
Testing Data
(Expression of calcitonin gene-related peptide (CGRP) and SNAP-25 in trigeminal ganglion neurons. The expression of CGRP (A) and SNAP-25 (B) are shown in the entire trigeminal ganglion obtained from an untreated adult animal.)
Testing Data
(Expression of calcitonin gene-related peptide (CGRP) and SNAP-25 in trigeminal ganglion neurons. The expression of CGRP (A) and SNAP-25 (B) are shown in the entire trigeminal ganglion obtained from an untreated adult animal.)
Product Categories/Family for anti-CALCB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
calcitonin gene-related peptide 2
NCBI Official Symbol
CALCB
NCBI Official Synonym Symbols
CGRP
NCBI Protein Information
calcitonin gene-related peptide 2; calcitonin-related polypeptide, beta
Protein Family
Research Articles on CALCB
Similar Products
Product Notes
The CALCB (Catalog #AAA615119) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The Calcitonin Gene-Related Peptide (CGRP) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Calcitonin Gene-Related Peptide can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC). Suitable for use in Immunohistochemistry. Dilution: Immunohistochemistry: 1:100-1:500. Used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode's solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at RT. C0115-01G was diluted with blocking buffer. Blocking buffer was removed from slides and they were then incubated for 18-24 hours at 4ºC with diluted C0115-01G. Slides were rinsed 3x and then incubated with secondary antibody for 1 hour at RT. Slides were again rinsed 3x and coverslipped. Staining was examined using fluorescence microscopy. Researchers should empirically determine the suitability of the CALCB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calcitonin Gene-Related Peptide, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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