Immunofluorescence (IF)
(IHC staining of rat spinal cord dorsal horn: Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode’s solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4oC with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy.)
Rabbit anti-Mouse, Rat Opioid Receptor 1C, mu Polyclonal Antibody
Opioid Receptor 1C, mu (MOR1C)
Reactivity
Mouse, Rat
Applications
Immunohistochemistry
Purity
SerumSerum
Synonyms
Opioid Receptor 1C; mu; Polyclonal Antibody; mu (MOR1C); Anti -Opioid Receptor 1C; anti-Opioid Receptor 1C; mu antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes rat MOR-1C. Species Crossreactivity: mouse.
Purity/Purification
Serum
Serum
Serum
Form/Format
Supplied as a liquid in 0.05% sodium azide.
Applicable Applications for anti-Opioid Receptor 1C, mu antibody
Immunohistochemistry (IHC)
Application Notes
Suitable for use in Immunohistochemistry.
Dilution: Immunohistochemistry: 1:5000-1:10000. Use perfusion fixed tissues. Perfusion: Calcium free Tyrodes solution, paraformaldehyde-picric acid fixative, and 10% sucrose in PBS as a cryo-protectant.
Dilution: Immunohistochemistry: 1:5000-1:10000. Use perfusion fixed tissues. Perfusion: Calcium free Tyrodes solution, paraformaldehyde-picric acid fixative, and 10% sucrose in PBS as a cryo-protectant.
Immunogen
Synthetic peptide corresponding to the aa. sequence KSCMDRGMRNLLPDDGPRQE.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunofluorescence (IF)
(IHC staining of rat spinal cord dorsal horn: Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode’s solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4oC with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy.)
Immunofluorescence (IF)
(IHC staining of rat spinal cord dorsal horn: Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode’s solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4oC with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy.)
Testing Data
(A-D: Staining of Differentiated Stem Cells. D3 ES cells were maintained in serum/LIF/ME induced self-renewal conditions (undifferentiated cells, D3). Upon treatment with uM RA they undergo differentiation (dD3). Cells were fixed and permeabilized. Treatments were followed by overnight incubation with MBS615891 at 1:2500 dilution. Reference: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2587057/pdf/nihms57104.pdf)
Testing Data
(A-D: Staining of Differentiated Stem Cells. D3 ES cells were maintained in serum/LIF/ME induced self-renewal conditions (undifferentiated cells, D3). Upon treatment with uM RA they undergo differentiation (dD3). Cells were fixed and permeabilized. Treatments were followed by overnight incubation with MBS615891 at 1:2500 dilution. Reference: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2587057/pdf/nihms57104.pdf)
Related Product Information for anti-Opioid Receptor 1C, mu antibody
Three types of opioid receptors have been cloned -- mu, delta, and kappa. Opioid receptors are seven transmembrane G-protein coupled receptors. They share a high degree of homology and are most divergent at the N- and C-termini. Activation of mu opioid receptors leads to a decrease in neuronal excitability. Several C-terminal splice variants have been identified for the Mu Opioid Receptor (MOR1) including MOR1C. These splice variants appear to differ in their pharmacological selectivity as well as their membrane trafficking.
Product Categories/Family for anti-Opioid Receptor 1C, mu antibody
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Product Notes
The Opioid Receptor 1C, mu (Catalog #AAA615891) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Opioid Receptor 1C, mu (MOR1C) reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Opioid Receptor 1C, mu can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC). Suitable for use in Immunohistochemistry. Dilution: Immunohistochemistry: 1:5000-1:10000. Use perfusion fixed tissues. Perfusion: Calcium free Tyrodes solution, paraformaldehyde-picric acid fixative, and 10% sucrose in PBS as a cryo-protectant. Researchers should empirically determine the suitability of the Opioid Receptor 1C, mu for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Opioid Receptor 1C, mu, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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