Rabbit Cadherin, pan Polyclonal Antibody | anti-cad antibody

Cadherin, pan

Cat. Num. AAA612062

• Gene Names: cad; 38E.19; anon-WO2004063362.83; Cad; CAD; cd; CG1759; DmelCG1759; S67
• Reactivity: Bovine, Canine, Chicken, Human, Monkey, Mouse, Rat, Xenopus
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Cadherin; pan; Polyclonal Antibody; Anti -Cadherin; anti-cad antibody

• Host: Rabbit
• Reactivity: Bovine, Canine, Chicken, Human, Monkey, Mouse, Rat, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This peptide antibody is broadly crossreactive with all members of the cadherin family of proteins including N-cadherin, E-cadherin, P-cadherin and R-cadherin. Rabbit anti-pan-Cadherin is useful as both a ubiquitous cadherin probe, as well as a marker for adherens junctions. Species crossreactivity: This antibody displays broad species crossreactivity including human, bovine, mouse, rat, chicken, amphibian (Xenopus), canine, monkey, as well as other species.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.

• Applicable Applications for anti-cad antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Immunohistochemistry: 1-10ug/ml. For Formalin fixed, paraffin embedded sections, Heat Induced Epitope Retrieval (HIER) step is required prior to staining.; Western Blot: 1ug/ml; ELISA: 0.1-1ug/ml
• Immunogen: Synthetic peptide derived from the C-terminus of the chicken N-cadherin protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-cad antibody

Cadherins are a multifunctional family of Ca2+ -dependent, transmembrane glycoproteins which promote and maintain cell adhesion in virtually all multicellular organisms. The cadherin superfamily comprises over forty proteins which are, on average, 50-60% homologous (1). Cadherin expression is required for the assembly of cells into solid tissues and importantly, cadherins are expressed in a tissue speci c fashion (2). Homotypic cellular interactions are promoted by homophillic interactions between the extracellular regions of identical cadherin molecules on neighboring cells. Recent crystal structure analysis of an extracellular cadherin domain suggests that individual cadherin molecules cooperate to form a linear cell adhesion zipper (3). In adherens junctions, cadherins are anchored to the actin cytoskeleton by interaction with the small cytoplasmic proteins b-catenin and g-catenin which both bind to the actin binding protein a-catenin (4,5). The interaction of b-catenin with the cytoplasmic tail of cadherins and other cytoplasmic proteins, including Tcf-family transcription factors and the tumor suppressor protein APC, is thought to be mediated through a region of the b-catenin molecule containing multiple repeats of the 42 amino acid armadillo sequence motif (6). In addition to playing important roles in differentiation and tissue morphogenesis, cadherins also appear to play a signi cant role in modulating tumor invasion and metastasis (7). The expression of E-cadherin correlates inversely with the motile and invasive behavior of tumor cells. In addition, the tissue speci city of cadherin subtypes are becoming valuable markers for the identi cation and differential identification of certain cancers (8,9).

Product Categories/Family for anti-cad antibody

Antibodies; Abs to Cadherin

NCBI and Uniprot Product Information

• NCBI GI #: 442628651
• NCBI GeneID: 35341
• NCBI Accession #: NP_001260643.1
• NCBI GenBank Nucleotide #: NM_001273714.1
• UniProt Accession #: P09085; Q9VIJ6; A4V0Y1; D4G7D2
• Molecular Weight: 45,726 Da
• NCBI Official Full Name: caudal, isoform E
• NCBI Official Synonym Full Names: caudal
• NCBI Official Symbol: cad
• NCBI Official Synonym Symbols: 38E.19; anon-WO2004063362.83; Cad; CAD; cd; CG1759; DmelCG1759; S67
• NCBI Protein Information: CG1759 gene product from transcript CG1759-RC; CG1759-PA; CG1759-PB; CG1759-PC; CG1759-PD; CG1759-PE; cad-PA; cad-PB; cad-PC; cad-PD; cad-PE; cadherin; caudal
• UniProt Protein Name: Homeotic protein caudal
• Protein Family: CAD protein
• UniProt Gene Name: cad
• UniProt Entry Name: CAD_DROME

Uniprot Description

Function: Caudal (cad) is one of a number of transcription factors controlling segmentation of the embryo. Further transcriptional regulation via a 5' flanking region containing DNA replication-related elements (DRE) and by dref also regulated by trh and tgo via the CNS midline element. Alongside Bicoid (bcd), caudal forms concentration gradients down the anterior-posterior (A-P) axis providing positional information and subsequent induction of the gap genes. Plays a role in gastrulation/germ band extension, hindgut morphogenesis, positive regulation of cell proliferation, genital disk development and pattern formation. Acts as a key regulator of the Hox gene network and activates transcription via the downstream core promoter element (DPE) relative to the TATA box. Plays a role in the establishment of the hindgut and in the invagination of the hindgut primordium during gastrulation. These effects on the gut are achieved by acting combinatorially at the posterior of the embryo to activate transcription of different targets including fog, fkh and wg. Caudal is involved in regulation of proliferation through transactivation of the E2F gene. Postembryonically its function is mostly restricted to the intestine where it regulates antimicrobial peptide (AMP) levels preserving the normal gut flora. Ref.7 Ref.8 Ref.9 Ref.10

Subcellular location: Nucleus Ref.1.

Tissue specificity: Maternally localized in an anteroposterior gradient in the syncytial blastoderm. Also expressed in the pole cells. Zygotically localized in the primordia of the terminal abdominal segment, the hindgut and in the posterior midgut rudiment. Expressed in the gut, the gonads and parts of the genital disks of third instar larvae (at protein level). Ref.1

Developmental stage: Expressed both maternally and zygotically. Ref.1 Ref.6

Sequence similarities: Belongs to the Caudal homeobox family.Contains 1 homeobox DNA-binding domain.

Sequence caution: The sequence AAA28409.1 differs from that shown. Reason: Frameshift at positions 44 and 418.