Rabbit anti-Human Cadherin 17/CDH17 Polyclonal Antibody | anti-CDH17 antibody

Anti-Cadherin 17/CDH17 Antibody, Rabbit Polyclonal

Cat. Num. AAA8105290

• Gene Names: CDH17; HPT1; CDH16; HPT-1
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: Cadherin 17/CDH17; Polyclonal Antibody; Anti-Cadherin 17/CDH17 Antibody; Rabbit Polyclonal; Cadherin-17/LI-cadherin/CDH17 Antibody; Rabbit PAb; Antigen Affinity Purified; cadherin 17; LI cadherin (liver-intestine); Anti-CDH16 Antibody; Anti-HPT-1 Antibody; Anti-HPT1 Antibody; anti-CDH17 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CDH17/Cadherin-17
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CDH17 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000; IHC-P: 1:2000-1:10,000; IP: 1-2uL/mg of lysate
• Immunogen: Recombinant Human CDH17/Cadherin-17 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human CDH17/Cadherin-17 (rh CDH17/Cadherin-17). CDH17/Cadherin-17 specific IgG was purified by Human CDH17/Cadherin-17 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human CDH17 in human rectal carcinoma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing membrane staining of intestinal gland. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)

Immunohistochemistry (IHC)

(Immunochemical staining of human CDH17 in human colon carcinoma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing membrane staining of colonic gland. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)

Immunohistochemistry (IHC)

(Immunochemical staining of human CDH17 in human appendix with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing membrane staining of intestinal gland.)

Immunoprecipitation (IP)

(CDH17 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate0.5 uL anti-CDH17 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CDH17 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 92 kDaObserved band size: 92 kDa)

Western Blot (WB)

(Anti-CDH17 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:92 kDaObserved band size:80 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-CDH17 antibody

Cadherin-17 or LI-cadherin is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. Cadherin-17/LI-cadherin is a cadherin-like protein consisting of an extracellular region, 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine. Alternative splicing of the encoding gene results in multiple transcript variants. Cadherin-17/LI-cadherin preferentially interact with themselves in a homophilic manner in connecting cells. Cadherin-17 may thus contribute to the sorting of heterogeneous cell types and have a role in the morphological organization of liver and intestine. It's also involved in intestinal peptide transport. Experiments have reported the association between Cadherin-17/LI-cadherin and gastric cancer. Cadherin-17/LI-cadherin expression was detected in 63/94 of gastric adenocarcinomas in addition to intestinal metaplasia. The expression of Cadherin-17 tended to be associated with intestinal type carcinoma, and carcinomas with Cadherin-17 expression was significantly more frequent in advanced stage cases than in early stage. Cadherin-17 is also a useful immunohistochemical marker for identification of adenocarcinomas of the digestive system.

Product Categories/Family for anti-CDH17 antibody

Canonical Wnt Pathway

References

Liu LX, et al. (2009) Targeting cadherin-17 inactivates Wnt signaling and inhibits tumor growth in liver carcinoma. Hepatology. 50(5): 1453-63.Ito R, et al. (2005) Clinicopathological significant and prognostic influence of cadherin-17 expression in gastric cancer. Virchows Arch. 447(4): 717-22.Horsfield J, et al. (2002) Cadherin-17 is required to maintain pronephric duct integrity during zebrafish development. Mech Dev. 115(1-2): 15-26.

NCBI and Uniprot Product Information

• NCBI GI #: 221316595
• NCBI GeneID: 1015
• NCBI Accession #: NP_001138135.1
• NCBI GenBank Nucleotide #: NM_001144663.1
• UniProt Accession #: Q12864; Q15336; Q2M2E0
• Molecular Weight: 92,219 Da
• NCBI Official Full Name: cadherin-17
• NCBI Official Synonym Full Names: cadherin 17, LI cadherin (liver-intestine)
• NCBI Official Symbol: CDH17
• NCBI Official Synonym Symbols: HPT1; CDH16; HPT-1
• NCBI Protein Information: cadherin-17; LI cadherin; LI-cadherin; cadherin-16; HPT-1 cadherin; liver-intestine cadherin; human peptide transporter 1; intestinal peptide-associated transporter HPT-1; human intestinal peptide-associated transporter HPT-1
• UniProt Protein Name: Cadherin-17
• Protein Family: Cadherin
• UniProt Gene Name: CDH17
• UniProt Synonym Gene Names: LI-cadherin
• UniProt Entry Name: CAD17_HUMAN

NCBI Description

This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009]

Uniprot Description

CDH17: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the morphological organization of liver and intestine. Involved in intestinal peptide transport.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 8q22.1

Cellular Component: basolateral plasma membrane; integral to membrane; plasma membrane

Molecular Function: transporter activity; calcium ion binding; proton-dependent oligopeptide secondary active transmembrane transporter activity

Biological Process: intercellular junction assembly and maintenance; transport; calcium-dependent cell-cell adhesion; oligopeptide transport; homophilic cell adhesion; cell adhesion