Testing Data
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Rabbit CAD Polyclonal Antibody | anti-CAD antibody
CAD antibody
Gene Names
DFFB; CAD; CPAN; DFF2; DFF40; DFF-40
Applications
Western Blot
Purity
Purified by Protein A affinity chromatography
Synonyms
CAD; Polyclonal Antibody; CAD antibody; Rabbit polyclonal CAD antibody; Polyclonal CAD antibody; Anti-CAD antibody; DFFB antibody; DNA fragmentation factor antibody; 40kDa antibody; beta polypeptide (caspase-activated DNase) antibody; BOS_15888 antibody; Cad antibody; caspase-activated deoxyribonuclease antibody; caspase-activated DNase antibody; caspase-activated nuclease antibody; CH211-147A11.2 antibody; CPAN antibody; DFF2 antibody; DFF40 antibody; DFF-40 antibody; Didff antibody; DNA fragmentation factor 40 kDa subunit antibody; 40 kD antibody; beta polypeptide antibody; DNA fragmentation factor 40 kD beta polypeptide (caspase-activated DNase) antibody; beta subunit antibody; DNA fragmentation factor subunit beta antibody; DNA fragmentation factor subunit beta-like antibody; DNase inhibited by DNA fragmentation factor antibody; RP13-531C17.1 antibody; RP23-254N4.2 antibody; anti-CAD antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A affinity chromatography
Form/Format
Liquid form in HEPES buffer with 0.15M NaCl,0.01% BSA,0.03% sodium azide, and 50% glycerol
Sequence Length
338
Applicable Applications for anti-CAD antibody
Western Blot (WB)
Application Notes
WB: 1:2000
Immunogen
CAD antibody was raised in Rabbit using a synthetic target peptide as the immunogen
Cross-Reactivity
Human
Preparation and Storage
Aliquot and store for 1 year at-20 degree C.
Avoid freeze/thaw cycles.
Avoid freeze/thaw cycles.
Testing Data
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Testing Data
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Related Product Information for anti-CAD antibody
CAD (caspase-activated DNase), a 40 kDa nuclear protein, is primarily responsible for cell-autonomous DNA degradation during apoptosis. CAD is present in healthy cells where it is held in an inactive state through the association with its inhibitor ICAD. The ICAD protein is inactivated in apoptotic cells via caspase-3 cleavage thereby releasing CAD, which subsequently cleaves chromosomal DNA. CAD is a magnesium-dependent endonuclease specific for double stranded DNA that generates double strand breaks with 3'-hydroxyl ends. The nuclease preferentially attacks chromatin in the internucleosomal linker DNA. However, the nuclease hypersensitive sites can be detected and CAD is potentially involved in large-scale DNA fragmentation as well. CAD-mediated DNA fragmentation triggers chromatin condensation that is another hallmark of apoptosis.
Product Categories/Family for anti-CAD antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
11,482 Da
NCBI Official Full Name
CAD
NCBI Official Synonym Full Names
DNA fragmentation factor subunit beta
NCBI Official Symbol
DFFB
NCBI Official Synonym Symbols
CAD; CPAN; DFF2; DFF40; DFF-40
NCBI Protein Information
DNA fragmentation factor subunit beta
UniProt Protein Name
DNA fragmentation factor subunit beta
Protein Family
UniProt Gene Name
DFFB
UniProt Synonym Gene Names
CAD; DFF2; DFF40; CAD; Caspase-activated DNase; CPAN; DFF-40
NCBI Description
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined. [provided by RefSeq, Sep 2013]
Uniprot Description
Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.
Research Articles on CAD
Similar Products
Product Notes
The CAD dffb (Catalog #AAA5310039) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CAD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:2000. Researchers should empirically determine the suitability of the CAD dffb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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