Rabbit anti-Human SMC1 Polyclonal Antibody | anti-SMC1 antibody

SMC1 (Phospho-Ser957) Antibody

Cat. Num. AAA9404807

• Gene Names: SMC1A; SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; SMC1alpha
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: SMC1; Polyclonal Antibody; SMC1 (Phospho-Ser957) Antibody; SM1A; SMC1A; SMC1L1; SMC1 alpha protein; anti-SMC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of SMC1 only when phosphorylated at serine 957.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 1233

• Applicable Applications for anti-SMC1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500~1:1000; Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of serine 957 (G-S-S(p)-Q-G) derived from Human SMC1.
• Modification: Phospho-Ser957
• Target Name: SMC1
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Western blot analysis of extracts from 293 cells untreated or treated with UV using SMC1(Phospho-Ser957) Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using SMC1(Phospho-Ser957) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

Related Product Information for anti-SMC1 antibody

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

Product Categories/Family for anti-SMC1 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 30581135
• NCBI GeneID: 8243
• NCBI Accession #: NP_006297.2
• NCBI GenBank Nucleotide #: NM_006306.3
• UniProt Accession #: Q14683; O14995; Q16351; Q2M228
• Molecular Weight: 143,233 Da
• NCBI Official Full Name: structural maintenance of chromosomes protein 1A isoform 1
• NCBI Official Synonym Full Names: structural maintenance of chromosomes 1A
• NCBI Official Symbol: SMC1A
• NCBI Official Synonym Symbols: SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; SMC1alpha
• NCBI Protein Information: structural maintenance of chromosomes protein 1A
• UniProt Protein Name: Structural maintenance of chromosomes protein 1A
• UniProt Gene Name: SMC1A
• UniProt Synonym Gene Names: DXS423E; KIAA0178; SB1.8; SMC1; SMC1L1; SMC protein 1A; SMC-1-alpha; SMC-1A
• UniProt Entry Name: SMC1A_HUMAN

NCBI Description

Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

Smc1: a component of the cohesin multiprotein complex that is required for sister chromatid cohesion. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Thought to be an important part of functional kinetochores. Interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair.

Protein type: RNA splicing; DNA replication

Chromosomal Location of Human Ortholog: Xp11.22-p11.21

Cellular Component: kinetochore; nucleoplasm; condensed nuclear chromosome; cohesin core heterodimer; cytoplasm; nucleolus; chromosome; nucleus; cytosol; chromosome, pericentric region

Molecular Function: protein binding; protein heterodimerization activity; chromatin binding; microtubule motor activity; ATP binding

Biological Process: nuclear mRNA splicing, via spliceosome; meiosis; response to radiation; mitotic spindle organization and biogenesis; mitotic sister chromatid segregation; cell division; mitotic cell cycle checkpoint; RNA splicing; stem cell maintenance; mitotic sister chromatid cohesion; sister chromatid cohesion; DNA damage response, signal transduction; negative regulation of DNA endoreduplication; gene expression; mitotic cell cycle; DNA repair

Disease: Cornelia De Lange Syndrome 2