Testing Data
TNFRSF1A elisa pair set
TNFRSF1A Matched ELISA Antibody Pair Set, Mouse
Synonyms
TNFRSF1A; TNFRSF1A Matched ELISA Antibody Pair Set; Mouse; Mouse TNFRSF1A ELISA Pair Set; tumor necrosis factor receptor superfamily member 1A; CD120a Matched ELISA Antibody Pair Set; FPF Matched ELISA Antibody Pair Set; p55 Matched ELISA Antibody Pair Set; p55-R Matched ELISA Antibody Pair Set; TNF-alphaR1 Matched ELISA Antibody Pair Set; TNF-R Matched ELISA Antibody Pair Set; TNF-R-I Matched ELISA Antibody Pair Set; TNF-R1 Matched ELISA Antibody Pair Set; TNF-R55 Matched ELISA Antibody Pair Set; TNFalpha-R1 Matched ELISA Antibody Pair Set; TNFAR Matched ELISA Antibody Pair Set; Tnfr-2 Matched ELISA Antibody Pair Set; Tnfr1 Matched ELISA Antibody Pair Set; TNFR60 Matched ELISA Antibody Pair Set; TNFRI Matched ELISA Antibody Pair Set; TNFRp55 Matched ELISA Antibody Pair Set; TNFRSF1A elisa pair set
Specificity
Quantitative determination of Mouse TNFRSF1A
Assay Type
Solid Phase Sandwich ELISA
Detection Range
4.69-300pg/mL
Sensitivity
4.69pg/ml
Materials Provided
1. Capture Antibody 0.3 mg/mL of rabbit anti-Mouse TNFRSF1A monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Mouse TNFRSF1A monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 21 ng of recombinant Mouse TNFRSF1A. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 300 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Mouse TNFRSF1A monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 21 ng of recombinant Mouse TNFRSF1A. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 300 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for TNFRSF1A elisa pair set
The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD120a (cluste of differentiation 120a), also known as TNFR1/TNFRSF1A, is a member of CD family, tumor necrosis factor receptor superfamily. CD120a is one of the most primary receptors for the tumor necrosis factor-alpha. It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD). CD120a can activate the transcription factor NF-kappaB, mediate apoptosis, and regulate inflammation processes.
This TNFRSF1A Matched ELISA Antibody Pair Set, Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse TNFRSF1A. It contains Mouse TNFRSF1A capture antibody, Mouse TNFRSF1A detector antibody (HRP) and a highly purified HEK293-expressed recombinant Mouse TNFRSF1A protein.
This TNFRSF1A Matched ELISA Antibody Pair Set, Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse TNFRSF1A. It contains Mouse TNFRSF1A capture antibody, Mouse TNFRSF1A detector antibody (HRP) and a highly purified HEK293-expressed recombinant Mouse TNFRSF1A protein.
Product Categories/Family for TNFRSF1A elisa pair set
References
Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.Cottin V, et al. (2002) Restricted localization of the TNF receptor CD120a to lipid rafts: a novel role for the death domain. The journal of immunology. 168: 4095-102.
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Product Notes
The TNFRSF1A (Catalog #AAA8118507) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "TNFRSF1A, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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