SDS-Page
(Detection of C4.4A with MBS619030. Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml MBS619030.)
Goat anti-Mouse C4.4A Polyclonal Antibody | anti-LYPD3 antibody
C4.4A (LYPD3, Y6/PLAUR domain containing 3 GPI-anchored metastasis-associated protein C4.4A homolog, Ly6/PLAUR domain-containing protein 3 precursor, Matrigel-induced gene C4 protein, MIG-C4, UNQ491/PRO1007)
Gene Names
LYPD3; C4.4A; LYPD3
Reactivity
Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
C4.4A; Polyclonal Antibody; C4.4A (LYPD3; Y6/PLAUR domain containing 3 GPI-anchored metastasis-associated protein C4.4A homolog; Ly6/PLAUR domain-containing protein 3 precursor; Matrigel-induced gene C4 protein; MIG-C4; UNQ491/PRO1007); Anti -C4.4A (LYPD3; anti-LYPD3 antibody
Host
Goat
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse C4.4A. In direct ELISA, this antibody shows approximately 40% cross-reactivity with rhC4.4A/LYPD3.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Applicable Applications for anti-LYPD3 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for Western Blot, Immunohistochemistry and ELISA. Other applicatons not tested.
Dilution: Western Blot: 1ug/ml. Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted.
Immunohistochemistry: 2-15ug/ml will detect C4.4A in cells and tissues.
Direct ELISA: 0.5-1ug/ml. The detection limit is ~0.3ng/well.
Optimal dilutions should be determined by each laboratory for each application.
Dilution: Western Blot: 1ug/ml. Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted.
Immunohistochemistry: 2-15ug/ml will detect C4.4A in cells and tissues.
Direct ELISA: 0.5-1ug/ml. The detection limit is ~0.3ng/well.
Optimal dilutions should be determined by each laboratory for each application.
Immunogen
Recombinant corresponding to mouse C4.4A expressed in NS0 cells (Q91YK8).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SDS-Page
(Detection of C4.4A with MBS619030. Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml MBS619030.)
SDS-Page
(Detection of C4.4A with MBS619030. Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml MBS619030.)
Related Product Information for anti-LYPD3 antibody
C4.4A (Ly6/PLAUR-domain containing protein 3; also MIG-C4 and GPI-anchored metastasis-associated protein C4.4A) is an 85-95kD, variably glycosylated member of the Ly6 family of GPI-anchored proteins. It is expressed on transitional epithelium, keratinocytes and monocytes, and binds laminin 1 and 5, plus Galectin-3. C4.4A is believed to support cell migration.
Product Categories/Family for anti-LYPD3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
35,971 Da
NCBI Official Full Name
C4.4A
NCBI Official Synonym Full Names
LY6/PLAUR domain containing 3
NCBI Official Symbol
LYPD3
NCBI Official Synonym Symbols
C4.4A; LYPD3
NCBI Protein Information
ly6/PLAUR domain-containing protein 3; MIG-C4; 2310061G07Rik; matrigel-induced gene C4 protein; GPI-anchored metastasis-associated protein homolog; GPI-anchored metastasis-associated protein C4.4A homolog
UniProt Protein Name
Ly6/PLAUR domain-containing protein 3
Protein Family
UniProt Gene Name
LYPD3
UniProt Synonym Gene Names
C4.4A
UniProt Entry Name
LYPD3_HUMAN
Uniprot Description
LYPD3: Supports cell migration. May be involved in urothelial cell-matrix interactions. May be involved in tumor progression.
Protein type: Motility/polarity/chemotaxis; Membrane protein, GPI anchor; Cell adhesion
Chromosomal Location of Human Ortholog: 19q13.31
Cellular Component: extracellular space; anchored to plasma membrane; integral to membrane
Molecular Function: laminin binding
Biological Process: cell-matrix adhesion
Research Articles on LYPD3
1. cleavage of C4.4A by ADAM10 and ADAM17 contributes to tumor progression
2. Overexpression of C4.4A is associated with invasion and metastasis of esophageal squamous cell carcinoma
3. High tumour cell C4.4A expression is associated with shorter survival for non-small cell lung cancer patients.
4. we consider C4.4A as a candidate diagnostic marker in colorectal cancer, which possibly can be detected in body fluids
5. hAG-2 and hAG-3, human homologues of genes involved in differentiation, are associated with oestrogen receptor-positive breast tumours and interact with metastasis gene C4.4a and dystroglycan
2. Overexpression of C4.4A is associated with invasion and metastasis of esophageal squamous cell carcinoma
3. High tumour cell C4.4A expression is associated with shorter survival for non-small cell lung cancer patients.
4. we consider C4.4A as a candidate diagnostic marker in colorectal cancer, which possibly can be detected in body fluids
5. hAG-2 and hAG-3, human homologues of genes involved in differentiation, are associated with oestrogen receptor-positive breast tumours and interact with metastasis gene C4.4a and dystroglycan
Similar Products
Product Notes
The LYPD3 lypd3 (Catalog #AAA619030) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The C4.4A (LYPD3, Y6/PLAUR domain containing 3 GPI-anchored metastasis-associated protein C4.4A homolog, Ly6/PLAUR domain-containing protein 3 precursor, Matrigel-induced gene C4 protein, MIG-C4, UNQ491/PRO1007) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's C4.4A can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for Western Blot, Immunohistochemistry and ELISA. Other applicatons not tested. Dilution: Western Blot: 1ug/ml. Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted. Immunohistochemistry: 2-15ug/ml will detect C4.4A in cells and tissues. Direct ELISA: 0.5-1ug/ml. The detection limit is ~0.3ng/well. Optimal dilutions should be determined by each laboratory for each application. Researchers should empirically determine the suitability of the LYPD3 lypd3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C4.4A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
