Rabbit GRP78 Polyclonal Antibody | anti-GRP78 antibody

GRP78 Antibody

Cat. Num. AAA8001986

• Gene Names: Hspa5; BIP; GRP78; GRP 78
• Reactivity: Human, Mouse, Rat, Bovine, Fungi, Chestnut Blight, Hamster, Monkey, Rabbit, African Clawed Frog, Dog
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Peptide Affinity Purified
• Synonyms: GRP78; Polyclonal Antibody; GRP78 Antibody; Rabbit Anti-Rat GRP78 Polyclonal; 78 kDa glucose regulated protein Antibody; 78 kDa glucose-regulated protein Antibody; AL022860 Antibody; AU019543 Antibody; BIP Antibody; D2Wsu141e Antibody; D2Wsu17e Antibody; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 Antibody; Endoplasmic reticulum lumenal Antibody; Ca2+ binding protein grp78 Antibody; FLJ26106 Antibody; Glucose Regulated Protein 78kDa Antibody; GRP 78 Antibody; GRP-78 Antibody; GRP78_HUMAN Antibody; Heat shock 70 kDa protein 5 Antibody; Heat Shock 70kDa Protein 5 Antibody; HSCe70 Antibody; HSPA 5 Antibody; HSPA5 Antibody; Immunoglobulin Heavy Chain Binding Protein Antibody; Immunoglobulin heavy chain-binding protein Antibody; mBiP Antibody; MIF2 Antibody; Sez7 Antibody; anti-GRP78 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Bovine, Fungi, Chestnut Blight, Hamster, Monkey, Rabbit, African Clawed Frog, Dog
• Clonality: Polyclonal
• Specificity: Detects ~78kDa.
• Purity/Purification: Peptide Affinity Purified
• Form/Format: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 654

• Applicable Applications for anti-GRP78 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:1000; ICC/IF: 1:100
• Immunogen: Rat GRP78 (Bip) synthetic peptide conjugated to KLH
• Immunogen Species: Rat
• Conjugate: Unconjugated
• Cellular Localization: Endoplasmic Reticulum, Endoplasmic Reticulum Lumen, Melanosome, Cytoplasm
• Certificate of Analysis: A 1:1000 dilution was sufficient for detection of Grp78 in 10 ug of rat tissue lysate by ECL immunoblot analysis.
• Preparation and Storage: Store at-20 degree C.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-GRP78 Polyclonal Antibody . Tissue: Hippocampal Section. Species: Mouse. Fixation: 4% Formaldehyde for 12 hours at RT. Paraffin embedded. Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody at 1:100 for 12 hours at 4 degree C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Rabbit at 1:250 for 1 hour at RT. Counterstain: Hoechst at 1:1000 for 10 min at RT. Localization: Grp78 staining in mouse pyramidal cell layer. Magnification: 20x. Slice thickness: 7 um. Courtesy of: Rachel Reith, NIH/NIMH.)

Western Blot (WB)

(Western blot analysis of Human, Dog, Mouse Cell line lysates showing detection of GRP78 protein using Rabbit Anti-GRP78 Polyclonal Antibody . Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody at 1:1000.)

Immunocytochemistry (ICC)/Immunofluorescence (IF)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP78 Polyclonal Antibody . Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody at 1:100 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP78 Antibody. (C) Composite. Heat Shocked at 42 degree C for 30 min.)

Immunocytochemistry (ICC)/Immunofluorescence (IF)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP78 Polyclonal Antibody . Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody at 1:100 for 12 hours at 4 degree C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP78 Antibody. (C) Composite. Heat Shocked at 42 degree C for 30 min.)

Western Blot (WB)

(Western blot analysis of Rat Tissue lysates showing detection of GRP78 protein using Rabbit Anti-GRP78 Polyclonal Antibody . Load: 15 ugprotein. Block: 1.5% BSA. Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)

Related Product Information for anti-GRP78 antibody

GRP78 is a ubiquitously expressed, 78-kDa glucose regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' non-translated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer's patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).

Product Categories/Family for anti-GRP78 antibody

Cancer; Heat Shock; Cell Signaling; Protein Trafficking; Chaperone Proteins; Tags and Cell Markers; Organelle Markers

References

1. Cho, S. et al. (2007). Mol Cell Biol 27(1): 368-83. 2. Yang, Y. et al. (1998) J Biol Chem 273: 25552-25555. 3. Luo, S. et al (2006) 26 (15): 5688-97. 4. Yu, Z. et al. (1999) Exp Neurol. 15: 302-314. 5. Koomagi, R. et al. (1999) Anticancer Res. 19:4333-4336. 6. Laquerre, S. et al. (1998) J. Virology 72: 4940-4949. 7. Dong, D. et al. (2005) Cancer Res 65(13): 5785-91.

NCBI and Uniprot Product Information

• NCBI GI #: 25742763
• NCBI GeneID: 25617
• NCBI Accession #: NP_037215.1
• NCBI GenBank Nucleotide #: NM_013083.2
• UniProt Accession #: P06761
• NCBI Official Full Name: endoplasmic reticulum chaperone BiP
• NCBI Official Synonym Full Names: heat shock protein family A member 5
• NCBI Official Symbol: Hspa5
• NCBI Official Synonym Symbols: BIP; GRP78; GRP 78
• NCBI Protein Information: endoplasmic reticulum chaperone BiP
• UniProt Protein Name: 78 kDa glucose-regulated protein
• Protein Family: 78 kDa glucose-regulated protein
• UniProt Gene Name: Hspa5
• UniProt Synonym Gene Names: Grp78; GRP-78; BiP

NCBI Description

secreted protein of the endoplasmic reticulum; may be involved in the assembly of secreted and membrane-bound proteins [RGD, Feb 2006]

Uniprot Description

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate ().