Rabbit anti-Human GNE Polyclonal Antibody | anti-GNE antibody

GNE (GLCNE, Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine Kinase, UDP-GlcNAc-2-epimerase/ManAc Kinase, UDP-N-acetylglucosamine 2-epimerase, UDP-GlcNAc-2-epimerase, Uridine Diphosphate-N-acetylglucosamine-2-epimerase, N-acetylmannosa

Cat. Num. AAA6380048

• Gene Names: GNE; NM; DMRV; IBM2; Uae1; GLCNE
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GNE; Polyclonal Antibody; GNE (GLCNE; Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine Kinase; UDP-GlcNAc-2-epimerase/ManAc Kinase; UDP-N-acetylglucosamine 2-epimerase; UDP-GlcNAc-2-epimerase; Uridine Diphosphate-N-acetylglucosamine-2-epimerase; N-acetylmannosa; anti-GNE antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GNE.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 3760

• Applicable Applications for anti-GNE antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human GNE, aa1-722 (NP_005467.1).
• Immunogen Sequence: MEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPEFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKSKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGIVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQAAKLGNAKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIY
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of GNE expression in transfected 293T cell line by GNE polyclonal antibody. Lane 1: GNE transfected lysate (79.3kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-GNE antibody

GNE is a bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. It is a rate-limiting enzyme in the sialic acid biosynthetic pathway. Sialic acid modification of cell surface molecules is crucial for their function in many biologic processes, including cell adhesion and signal transduction. Differential sialylation of cell surface molecules is also implicated in the tumorigenicity and metastatic behavior of malignant cells.

Product Categories/Family for anti-GNE antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 38788307
• NCBI GeneID: 10020
• NCBI Accession #: NM_005476.3
• NCBI GenBank Nucleotide #: NM_005476.3
• NCBI Official Full Name: Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), mRNA
• NCBI Official Synonym Full Names: glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
• NCBI Official Symbol: GNE
• NCBI Official Synonym Symbols: NM; DMRV; IBM2; Uae1; GLCNE
• NCBI Protein Information: bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
• Protein Family: Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase

NCBI Description

The protein encoded by this gene is a bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. It is a rate-limiting enzyme in the sialic acid biosynthetic pathway. Sialic acid modification of cell surface molecules is crucial for their function in many biologic processes, including cell adhesion and signal transduction. Differential sialylation of cell surface molecules is also implicated in the tumorigenicity and metastatic behavior of malignant cells. Mutations in this gene are associated with sialuria, autosomal recessive inclusion body myopathy, and Nonaka myopathy. Alternative splicing of this gene results in transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]