Mouse Chromosome 14 Open Reading Frame 169 Monoclonal Antibody | anti-C14orf169 antibody

Chromosome 14 Open Reading Frame 169 (C14orf169, FLJ21802)

Cat. Num. AAA600266

• Gene Names: C14orf169; NO66; MAPJD; FLJ21802
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Chromosome 14 Open Reading Frame 169; Monoclonal Antibody; Chromosome 14 Open Reading Frame 169 (C14orf169; FLJ21802); Anti -Chromosome 14 Open Reading Frame 169 (C14orf169; anti-C14orf169 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8C75
• Specificity: Recognizes human Chromosome 14 Open Reading Frame 169.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.

• Applicable Applications for anti-C14orf169 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blotting.; Dilution: Western Blotting: 1:1000-1:2000
• Immunogen: Partial sequence of recombinant full-length protein to human Chromosome 14 Open Reading Frame 169
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

Product Categories/Family for anti-C14orf169 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 15030188
• NCBI GeneID: 79697
• UniProt Accession #: Q9H6W3; B4DT02
• Molecular Weight: 71,086 Da
• NCBI Official Full Name: Chromosome 14 open reading frame 169
• NCBI Official Synonym Full Names: chromosome 14 open reading frame 169
• NCBI Official Symbol: C14orf169
• NCBI Official Synonym Symbols: NO66; MAPJD; FLJ21802
• NCBI Protein Information: lysine-specific demethylase NO66; hsNO66; OTTHUMP00000233252; nucleolar protein 66; MYC-associated protein with JmjC domain
• UniProt Protein Name: Lysine-specific demethylase NO66
• UniProt Gene Name: NO66
• UniProt Synonym Gene Names: C14orf169
• UniProt Entry Name: NO66_HUMAN

Uniprot Description

NO66: Histone demethylase that specifically demethylates 'Lys- 4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation. May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC-induced transcriptional activation. Belongs to the MINA53/NO66 family. NO66 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; EC 1.14.11.27

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: histone demethylase activity (H3-K4 specific); protein binding; iron ion binding; histone demethylase activity (H3-K36 specific); oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors

Biological Process: transcription, DNA-dependent; negative regulation of osteoblast differentiation; negative regulation of transcription, DNA-dependent