Rabbit anti-Human c-Met Polyclonal Antibody | anti-MET antibody

c-Met, CT (BSA, Azide and Glycerol Free) (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR)

Cat. Num. AAA6010708

• Gene Names: MET; c-Met
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: c-Met; Polyclonal Antibody; CT (BSA; Azide and Glycerol Free) (Hepatocyte Growth Factor Receptor; Scatter Factor Receptor; HGFR; SFR); Anti -c-Met; anti-MET antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes the 145kD human c-Met protein. Does not crossreact with related proteins.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4. No stabilizing proteins, no preservatives added. Glycerol free. Available with azide, BSA and glycerol. See M3007-13.

• Applicable Applications for anti-MET antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA (Immunogen): 0.1-1ug/ml; Western Blot: 1-2ug/ml; Immunohistochemistry (Paraffin): 1-2ug/ml. Best results obtained by subjecting tissue to heat-induced epitope retrieval (HIER).
• Immunogen: Synthetic peptide corresponding to 13aa located within last 50aa derived from the C-terminus of the human c-Met protein.
• Positive Control: Western Blot: A431 cell extracts; Immunohistochemistry: Human breast cancer tissue
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-MET antibody

Following its characterization as an activated oncogene (based on its ability to transform normal fibroblasts) Met was characterized as the receptor for hepatocyte growth factor. The c-Met receptor is a disulfide bonded heterodimer consisting of an ~50kD extracellular a-chain and an ~145kD b-chain with a cytoplasmic tyrosine kinase domain. Both chains are derived from a single precursor protein by proteolytic cleavage. c-Met is expressed in a wide variety of adult and embryonic tissues. It transmits biological responses including mitogenesis, morphogenesis and anti-apoptotic activity elicited by HGF. Dysregulated c-Met gene expression is observed in a variety of human carcinomas and sarcomas. c-Met also mediates the movement and invasiveness of neoplastic cells and promotes metastasis. Recently, transcription of the c-Met gene was suggested to be a target for regulation by p53 (1).

Product Categories/Family for anti-MET antibody

Antibodies; Abs to Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 25815188
• NCBI GeneID: 403438
• UniProt Accession #: Q75ZY9; A0M8U9
• Molecular Weight: 154,637 Da
• NCBI Official Full Name: c-Met
• NCBI Official Symbol: MET
• NCBI Official Synonym Symbols: c-Met
• NCBI Protein Information: hepatocyte growth factor receptor; SF receptor; HGF receptor; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogene tyrosine kinase
• UniProt Protein Name: Hepatocyte growth factor receptor
• Protein Family: C-methyltransferase
• UniProt Gene Name: MET
• UniProt Synonym Gene Names: HGF receptor; SF receptor
• UniProt Entry Name: MET_CANFA

Uniprot Description

Function: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells

By similarity. Ref.4

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Enzyme regulation: In its inactive state, the C-terminal tail interacts with the catalytic domain and inhibits the kinase activity. Upon ligand binding, the C-terminal tail is displaced and becomes phosphorylated, thus increasing the kinase activity

By similarity.

Subunit structure: Heterodimer made of an alpha chain (50 kDa) and a beta chain (145 kDa) which are disulfide linked. Binds PLXNB1. Interacts when phosphorylated with downstream effectors including STAT3, PIK3R1, SRC, PCLG1, GRB2 and GAB1. Interacts with SPSB1, SPSB2 and SPSB4. Interacts with INPP5D/SHIP1. When phosphorylated at Tyr-1357, interacts with INPPL1/SHIP2. Interacts with RANBP9 and RANBP10, as well as SPSB1, SPSB2, SPSB3 and SPSB4. SPSB1 binding occurs in the presence and in the absence of HGF, however HGF treatment has a positive effect on this interaction. Interacts with MUC20; prevents interaction with GRB2 and suppresses hepatocyte growth factor-induced cell proliferation. Interacts with GRB10

By similarity. Interacts with PTPN1 and PTPN2

By similarity.

Subcellular location: Membrane; Single-pass type I membrane protein

By similarity.

Domain: The kinase domain is involved in SPSB1 binding

By similarity.The beta-propeller Sema domain mediates binding to HGF

By similarity.

Post-translational modification: Autophosphorylated in response to ligand binding on Tyr-1235 and Tyr-1236 in the kinase domain leading to further phosphorylation of Tyr-1350 and Tyr-1357 in the C-terminal multifunctional docking site. Dephosphorylated by PTPRJ at Tyr-1350 and Tyr-1366. Dephosphorylated by PTPN1 and PTPN2

By similarity.Ubiquitinated. Ubiquitination by CBL regulates the receptor stability and activity through proteasomal degradation

By similarity.

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family.Contains 3 IPT/TIG domains.Contains 1 protein kinase domain.Contains 1 Sema domain.