Western Blot (WB)
(Western Blot: Sample: Recombinant IREM1, Mouse.)
Rabbit anti-Mouse Immune Receptor Expressed On Myeloid Cells 1 Polyclonal Antibody | anti-IREM1 antibody
Polyclonal Antibody to Immune Receptor Expressed On Myeloid Cells 1 (IREM1)
Gene Names
Cd300lf; CLM1; CLIM1; CLM-1; Digr2; IREM1; LMIR3; Pigr3; IgSF13; F730004D16Rik
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Immune Receptor Expressed On Myeloid Cells 1; Polyclonal Antibody; Polyclonal Antibody to Immune Receptor Expressed On Myeloid Cells 1 (IREM1); anti-IREM1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against IREM1. It has beenselected for its ability to recognize IREM1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-SGWKDY KKYWCQGVPQ RSCKTLVETD ASEQLVKKNR VSIRDNQRDF IFTVTMEDLR MSDAGIYWCG ITKGGLDPMF KVTVNIGPAI QVPITVPTMP PITSTTTIFT VTTTVKETSM FPTLTSYYSD NGHGGGDSGG GEDGVGDGFL DLSVLLPVIS AVLLLLLLVA SLFAWRMVRR QKKAAGPPSE QAQSLEGDLC YADLSLKQPR TSPGSSWKKG SSMSSSGKDH QEEVEYVTMA
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-SGWKDY KKYWCQGVPQ RSCKTLVETD ASEQLVKKNR VSIRDNQRDF IFTVTMEDLR MSDAGIYWCG ITKGGLDPMF KVTVNIGPAI QVPITVPTMP PITSTTTIFT VTTTVKETSM FPTLTSYYSD NGHGGGDSGG GEDGVGDGFL DLSVLLPVIS AVLLLLLLVA SLFAWRMVRR QKKAAGPPSE QAQSLEGDLC YADLSLKQPR TSPGSSWKKG SSMSSSGKDH QEEVEYVTMA
Applicable Applications for anti-IREM1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Fragment
IREM1 (Ser45~Ile310)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Human, Mouse
Conjugate
No Conjugate
Immunogen
Recombinant IREM1 (Ser45~Ile310) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2054566
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant IREM1, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant IREM1, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,791 Da
NCBI Official Full Name
CMRF35-like molecule 1 isoform 1
NCBI Official Synonym Full Names
CD300 molecule like family member F
NCBI Official Symbol
Cd300lf
NCBI Official Synonym Symbols
CLM1; CLIM1; CLM-1; Digr2; IREM1; LMIR3; Pigr3; IgSF13; F730004D16Rik
NCBI Protein Information
CMRF35-like molecule 1
UniProt Protein Name
CMRF35-like molecule 1
UniProt Gene Name
Cd300lf
UniProt Synonym Gene Names
Clm1; Lmir3; CLM-1; MAIR-5; MAIR-V
Uniprot Description
IREM-1: Acts as an inhibitory receptor for myeloid cells and mast cells. Inhibits osteoclast formation. Belongs to the CD300 family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Immunoglobulin superfamily; Membrane protein, integral; Receptor, misc.
Chromosomal Location of Human Ortholog: 11|11 E2
Cellular Component: integral component of membrane; membrane; plasma membrane
Molecular Function: interleukin-4 receptor binding; lipid binding; phosphatidylserine binding; protein binding; viral receptor activity
Biological Process: immune system process; negative regulation of mast cell activation; negative regulation of MyD88-dependent toll-like receptor signaling pathway; osteoclast differentiation
Research Articles on IREM1
Similar Products
Product Notes
The IREM1 cd300lf (Catalog #AAA2028064) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Immune Receptor Expressed On Myeloid Cells 1 (IREM1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Immune Receptor Expressed On Myeloid Cells 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the IREM1 cd300lf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-SGW KDY KKYWCQGVPQ RSCKTLVETD ASEQLVKKNR VSIRDNQRDF IFTVTMEDLR MSDAGIYWCG ITKGGLDPMF KVTVNIGPAI QVPITVPTMP PITSTTTIFT VTTTVKETSM FPTLTSYYSD NGHGGGDSGG GEDGVGDGFL DLSVLLPVIS AVLLLLLLVA SLFAWRMVRR QKKAAGPPSE QAQSLEGDLC YADLSLKQPR TSPGSSWKKG SSMSSSGKDH QEEVEYVTMA. It is sometimes possible for the material contained within the vial of "Immune Receptor Expressed On Myeloid Cells 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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