Western Blot (WB)
(Western blot analysis of extracts from HEK-293 cells untreated or treated with Anisomycin using Rabbit anti c-Jun (pSer63) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with Anisomycin (5ug/ml) for 30 minutes)
Rabbit anti-Human c-Jun Polyclonal Antibody | anti-JUN antibody
Rabbit anti c-Jun (pSer63)
Gene Names
JUN; AP1; p39; AP-1; c-Jun
Reactivity
Human
Applications
Western Blot
Purity
Purified whole serum by affinity chromatography.
Synonyms
c-Jun; Polyclonal Antibody; Rabbit anti c-Jun (pSer63); c-Jun (pSer63) Antibody; anti-JUN antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified whole serum by affinity chromatography.
Form/Format
Liquid in phosphate buffered saline with 0.02% sodium azide, 50% glycerol.
Sequence Length
331
Applicable Applications for anti-JUN antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 63 of human c-Jun
Preperation
Antiserum to c-Jun (pSer63) was raised by repeated immunization of rabbits with highly purified antigen.
Preparation and Storage
Store at -20 degree C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Western Blot (WB)
(Western blot analysis of extracts from HEK-293 cells untreated or treated with Anisomycin using Rabbit anti c-Jun (pSer63) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with Anisomycin (5ug/ml) for 30 minutes)
Western Blot (WB)
(Western blot analysis of extracts from HEK-293 cells untreated or treated with Anisomycin using Rabbit anti c-Jun (pSer63) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with Anisomycin (5ug/ml) for 30 minutes)
Related Product Information for anti-JUN antibody
Rabbit anti c-Jun (pSer63) antibody recognizes proto-oncogene c-Jun, also known as V-jun avian sarcoma virus 17 oncogene homolog and p39, when phosphorylated at serine 63.
c-Jun is a member of the Jun family of transcription factors and is a subunit of the AP-1 transcription factor. c-Jun dimerizes with c-Fos, JDP and ATF family members to form the AP-1 transcription factor. AP-1 is activated by a variety of stimuli including cytokines, stress and growth factors and plays a critical role in cellular processes ranging from proliferation to apoptosis.
c-Jun is phosphorylated at serine 63 and serine 73 by MAPK8 (SAPK1/JNK1) and PLK3, resulting in c-Jun mediated transcription (Wang et al. 2007, Vleugel et al. 2006). c-Jun has been implicated in early tumor development, invasion and metastasis while c-Jun phosphorylated at serine 63 has been proposed to play a role in intestinal tumor progression (Madeo et al. 2010).
c-Jun is a member of the Jun family of transcription factors and is a subunit of the AP-1 transcription factor. c-Jun dimerizes with c-Fos, JDP and ATF family members to form the AP-1 transcription factor. AP-1 is activated by a variety of stimuli including cytokines, stress and growth factors and plays a critical role in cellular processes ranging from proliferation to apoptosis.
c-Jun is phosphorylated at serine 63 and serine 73 by MAPK8 (SAPK1/JNK1) and PLK3, resulting in c-Jun mediated transcription (Wang et al. 2007, Vleugel et al. 2006). c-Jun has been implicated in early tumor development, invasion and metastasis while c-Jun phosphorylated at serine 63 has been proposed to play a role in intestinal tumor progression (Madeo et al. 2010).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
35,676 Da
NCBI Official Full Name
Transcription factor AP-1
NCBI Official Synonym Full Names
Jun proto-oncogene, AP-1 transcription factor subunit
NCBI Official Symbol
JUN
NCBI Official Synonym Symbols
AP1; p39; AP-1; c-Jun
NCBI Protein Information
transcription factor AP-1
UniProt Protein Name
Transcription factor AP-1
Protein Family
UniProt Gene Name
JUN
UniProt Synonym Gene Names
AP1
NCBI Description
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]
Uniprot Description
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
Research Articles on JUN
Similar Products
Product Notes
The JUN jun (Catalog #AAA225215) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti c-Jun (pSer63) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's c-Jun can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the JUN jun for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-Jun, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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