Human Aminoadipate Aminotransferase (AADAT) RTU ELISA Kit | AADAT rtu elisa kit

Human Aminoadipate Aminotransferase (AADAT) ELISA Kit

Cat. Num. AAA457280

• Gene Names: AADAT; KAT2; KATII; KYAT2
• Reactivity: Human
• Synonyms: Aminoadipate Aminotransferase (AADAT); Human Aminoadipate Aminotransferase (AADAT) ELISA Kit; KATII; KAT2; Kynurenine Aminotransferase II; L Kynurenine/Alpha Aminoadipate Aminotransferase; Kynurenine aminotransferase II; AADAT rtu elisa kit

• Reactivity: Human
• Sequence Length: 429

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.062ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AADAT rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AADAT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to AADAT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AADAT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AADAT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 557878723
• NCBI GeneID: 51166
• NCBI Accession #: NP_001273611.1
• NCBI GenBank Nucleotide #: NM_001286682.1
• UniProt Accession #: Q8N5Z0; Q9UL02; B3KP84
• Molecular Weight: 47,822 Da
• NCBI Official Full Name: kynurenine/alpha-aminoadipate aminotransferase, mitochondrial isoform a
• NCBI Official Synonym Full Names: aminoadipate aminotransferase
• NCBI Official Symbol: AADAT
• NCBI Official Synonym Symbols: KAT2; KATII; KYAT2
• NCBI Protein Information: kynurenine/alpha-aminoadipate aminotransferase, mitochondrial
• UniProt Protein Name: Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial
• Protein Family: Kynurenine/alpha-aminoadipate aminotransferase
• UniProt Gene Name: AADAT
• UniProt Synonym Gene Names: KAT2; KAT/AadAT; AadAT

NCBI Description

This gene encodes a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

Uniprot Description

Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro).