Rabbit anti-Human C-JUN Polyclonal Antibody | anti-C-JUN antibody

RABBIT ANTI C-JUN (pSer73)

Cat. Num. AAA224952

• Gene Names: JUN; AP1; AP-1; c-Jun
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Synonyms: C-JUN; Polyclonal Antibody; RABBIT ANTI C-JUN (pSer73); anti-C-JUN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: C-JUN (pSer73)
• Form/Format: Purified IgG - liquid
• Concentration: IgG concentration 0.5 mg/ml (varies by lot)
• Sequence Length: 331

• Applicable Applications for anti-C-JUN antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: Immunohistochemistry-Paraffin: 10; 20 ug/ml; Western Blot: 0.5; 4.0 ug/ml
• Reactivity Notes: Mouse, Rat
• Preservative Stabilisers: 50% Glycerol, 1% Bovine Serum Albumin, 0.02% Thiomersal
• Buffer Solution: Phosphate buffered saline
• Preparation and Storage: Store at -20 degree C Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. ; Shelf Life: 12 months from date of despatch

Western Blot (WB)

(Western blot analysis of NIH/3T3 cell lysate, treated with (right hand lane) or without (left hand lane) anisomycin, probed with Rabbit anti c-Jun (pSer73) antibody)

Related Product Information for anti-C-JUN antibody

Rabbit anti Human c-Jun (pSer 73) recognizes c-Jun also known as p39 phosphorylated on Serine residue 73. The c-Jun transcription factor is encoded by the c-jun oncogene and dimerizes with c-Fos, JDP and ATF family members to form the AP-1 transcription factor. AP-1 is activated by a variety of stimuli including cytokines, stress and growth factors and plays a critical role in cellular processes ranging from proliferation to apoptosis.c-Jun becomes phosphorylated on Serine 63 and Serine 73 by MAPK8 (SAPK/JNK) and PLK3, which regulate c-Jun transcription. c-Jun has been implicated in early tumor development, invasion and metastasis and due to these findings has been identified as a target for cancer therapies.

NCBI and Uniprot Product Information

• NCBI GI #: 135298
• NCBI GeneID: 3725
• UniProt Accession #: P05412; Q6FHM7; Q96G93
• Molecular Weight: 35,676 Da
• NCBI Official Full Name: Transcription factor AP-1
• NCBI Official Synonym Full Names: jun proto-oncogene
• NCBI Official Symbol: JUN
• NCBI Official Synonym Symbols: AP1; AP-1; c-Jun
• NCBI Protein Information: transcription factor AP-1
• UniProt Protein Name: Transcription factor AP-1
• Protein Family: C-Jun-amino-terminal kinase-interacting protein
• UniProt Gene Name: JUN
• UniProt Synonym Gene Names: AP1
• UniProt Entry Name: JUN_HUMAN

NCBI Description

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]

Uniprot Description

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).