Rabbit anti-Human, Mouse Bub1 Polyclonal Antibody | anti-BUB1 antibody

Bub1 Antibody

Cat. Num. AAA151094

• Gene Names: BUB1; BUB1A; BUB1L; hBUB1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Bub1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: Bub1; Polyclonal Antibody; Bub1 Antibody; BUB1A; BUB1L; hBUB1; budding uninhibited by benzimidazoles 1 homolog (yeast); anti-BUB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Bub1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1085

• Applicable Applications for anti-BUB1 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: Bub1 antibody can be used for detection of bub1 by Western blot at 2 - 4 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Bub1 antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human Bub1.
• Buffer: Bub1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Bub1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of bub1 in A-20 lysate with bub1 antibody at (A) 2 and (B) 4 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry of Bub1 in A20 cells with Bub1 antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of Bub1 in A20 cells with Bub1 antibody at 20 μg/mL.)

Related Product Information for anti-BUB1 antibody

Bub1 Antibody: Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen. Bub1 encodes a kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation. It is thought to be required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshim protein and metaphase congression. Bub1 functions by phosphorylating cdc20, a member of the mitotic checkpoint complex and activating the spindle checkpoint. A related protein kinase Bub3 interacts with Bub1 and targets it to kinetochores prior to chromosome alignment. Mutations in bub1 have been associated with aneuploidy and several forms of cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 8134347
• NCBI GeneID: 699
• UniProt Accession #: O43683; O43430; O43643; O60626; Q53QE4; E9PC26; F5GXI5
• Molecular Weight: 120,002 Da
• NCBI Official Full Name: Mitotic checkpoint serine/threonine-protein kinase BUB1
• NCBI Official Synonym Full Names: BUB1 mitotic checkpoint serine/threonine kinase
• NCBI Official Symbol: BUB1
• NCBI Official Synonym Symbols: BUB1A; BUB1L; hBUB1
• NCBI Protein Information: mitotic checkpoint serine/threonine-protein kinase BUB1; mitotic spindle checkpoint kinase; putative serine/threonine-protein kinase; budding uninhibited by benzimidazoles 1 homolog; BUB1 budding uninhibited by benzimidazoles 1 homolog
• UniProt Protein Name: Mitotic checkpoint serine/threonine-protein kinase BUB1
• Protein Family: Checkpoint serine/threonine-protein kinase
• UniProt Gene Name: BUB1
• UniProt Synonym Gene Names: BUB1L; hBUB1
• UniProt Entry Name: BUB1_HUMAN

NCBI Description

This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

BUB1: a mitotic-checkpoint protein kinase of the BUB family. Autophosphorylated when the cells enters mitosis. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage. High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index. LOF mutations seen in two colon cancer cell lines exhibiting chromosomal instability, in several leukemias and lymphomas, and in a pancreatic cancer cell line. BUB1 functions in mitotic spindle assembly, and may cause chromosomal instability. In mouse BRCA2 knockouts, mutations in BUB1 and BUBR1 are frequently seen in the resulting tumors. BUB1 and BUBR1 are overexpressed, but not mutated, in many gastric cancers.

Protein type: Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Cell cycle regulation; Kinase, protein; EC 2.7.11.1; Other group; BUB family

Chromosomal Location of Human Ortholog: 2q14

Cellular Component: nucleoplasm; kinetochore; membrane; cytoplasm; cytosol; condensed nuclear chromosome, pericentric region

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity

Biological Process: mitosis; cell proliferation; viral reproduction; apoptosis; cell division; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; regulation of sister chromatid cohesion; regulation of chromosome segregation; mitotic cell cycle; protein amino acid phosphorylation; chromosome segregation