Western Blot (WB)
(Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-3: K562 cell, Mouse brain tissue and RAW264.7 cell lysates, Primary antibody: 162942(BRI3BP Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds)
Rabbit BRN3B/POU4F2 Polyclonal Antibody | anti-BRN3B/POU4F2 antibody
BRN3B/POU4F2 Rabbit pAb
Gene Names
BRI3BP; KG19; BNAS1; HCCR-1; HCCR-2; HCCRBP-1; HCCRBP-3
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
BRN3B/POU4F2; Polyclonal Antibody; BRN3B/POU4F2 Rabbit pAb; KG19; BNAS1; HCCR-1; HCCR-2; HCCRBP-1; anti-BRN3B/POU4F2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-BRN3B/POU4F2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:200-1:1000
Immunogen
Synthetic peptide of human BRI3BP
Conjugation
Unconjugated
Molecular Function
BRI3BP (BRI3 binding protein), also known as BNAS1, HCCR-1, I3-binding protein or cervical cancer 1 proto-oncogene-binding protein KG19, is a 251 amino acid multi-pass membrane protein. Though widely expressed, BRI3BP is found at highest levels in brain, kidney and liver where it localizes to the endoplasmic reticulum (ER) and is involved in ER structural dynamics and mitochondrial viability. Possessing pro-apoptotic properties and the ability to potentiate drug-induced apoptosis, BRI3BP overexpression has been shown to enhance caspase-3 and mitochondrial cytochrome c release in etoposide-treated human embryonic kidney 293T cells.
Modification
Unmodified
Cell Position
Membrane, Mitochondrion, Mitochondrion outer membrane
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-3: K562 cell, Mouse brain tissue and RAW264.7 cell lysates, Primary antibody: 162942(BRI3BP Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds)
Western Blot (WB)
(Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-3: K562 cell, Mouse brain tissue and RAW264.7 cell lysates, Primary antibody: 162942(BRI3BP Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds)
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 162942(BRI3BP Antibody) at dilution 1/20. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 162942(BRI3BP Antibody) at dilution 1/20. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using 162942(BRI3BP Antibody) at dilution 1/20. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using 162942(BRI3BP Antibody) at dilution 1/20. (Original magnification: ×200))
Product Categories/Family for anti-BRN3B/POU4F2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28kDa
NCBI Official Full Name
BRI3-binding protein
NCBI Official Synonym Full Names
BRI3 binding protein
NCBI Official Symbol
BRI3BP
NCBI Official Synonym Symbols
KG19; BNAS1; HCCR-1; HCCR-2; HCCRBP-1; HCCRBP-3
NCBI Protein Information
BRI3-binding protein
UniProt Protein Name
BRI3-binding protein
UniProt Gene Name
BRI3BP
UniProt Synonym Gene Names
KG19; I3-binding protein
UniProt Entry Name
BRI3B_HUMAN
Uniprot Description
BRI3BP: Involved in tumorigenesis and may function by stabilizing p53/TP53.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Oncoprotein
Chromosomal Location of Human Ortholog: 12q24.31
Cellular Component: mitochondrial outer membrane; mitochondrion; integral to membrane
Research Articles on BRN3B/POU4F2
Similar Products
Product Notes
The BRN3B/POU4F2 bri3bp (Catalog #AAA4754965) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BRN3B/POU4F2 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BRN3B/POU4F2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:200-1:1000. Researchers should empirically determine the suitability of the BRN3B/POU4F2 bri3bp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRN3B/POU4F2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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