Rabbit BRN3B/POU4F2 Polyclonal Antibody | anti-POU4F2 antibody

BRN3B/POU4F2 Antibody

Cat. Num. AAA9629090

• Gene Names: POU4F2; BRN3B; BRN3.2; Brn-3b
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: BRN3B/POU4F2; Polyclonal Antibody; BRN3B/POU4F2 Antibody; Brain specific homeobox/POU domain protein 3B; Brain-3B; Brain-specific homeobox/POU domain protein 3B; Brn-3B; BRN3.2; BRN3B; Brn3b POU domain transcription factor; class 4; PO4F2_HUMAN; POU class 4 homeobox 2; POU domain; POU domain class 4 transcription factor 2; POU domain protein; POU4F2; transcription factor 2; anti-POU4F2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: BRN3B/POU4F2 Antibody detects endogenous levels of total BRN3B/POU4F2.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-POU4F2 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: The N-terminal transcriptional activation region is sufficient to induce transcriptional activity. The POU-specific domain and POU homeodomain regions are necessary for DNA-binding activity and transcriptional repression. The polyhistidine motif acts as a targeting signal to nuclear speckles. Belongs to the POU transcription factor family. Class-4 subfamily.
• Subunit Structure: Interacts with POU4F1; this interaction inhibits both POU4F1 DNA-binding and transcriptional activities. Interacts (C-terminus) with ESR1 (via DNA-binding domain); this interaction increases the estrogen receptor ESR1 transcriptional activity in a DNA- and ligand 17-beta-estradiol-independent manner. Interacts (via C-terminus) with TP53 (via N-terminus). Interacts with DLX1 (via homeobox DNA-binding domain); this interaction suppresses DLX1-mediated transcriptional activity in postnatal retina enhancing retinal ganglion cell (RGC) differentiation. Interacts with DLX2 (via homeobox DNA-binding domain); this interaction enhances RGC differentiation. Interacts (via C-terminus) with ISL1 (via C-terminus). Interacts with ISL2. Interacts with LHX2.
• Tissue Specificity: Expressed in the brain. Expressed in the ganglion cell layer of the retina.
• Function: Tissue-specific DNA-binding transcription factor involved in the development and differentiation of target cells. Functions either as activator or repressor modulating the rate of target gene transcription through RNA polymerase II enzyme in a promoter-dependent manner. Binds to the consensus octamer motif 5'-AT[A/T]A[T/A]T[A/T]A-3' of promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. Binds to an octamer site to form a ternary complex with ISL1; cooperates positively with ISL1 and ISL2 to potentiate transcriptional activation of RGC target genes being involved in RGC fate commitment in the developing retina and RGC axon formation and pathfinding. Inhibits DLX1 and DLX2 transcriptional activities preventing DLX1- and DLX2-mediated ability to promote amacrine cell fate specification. In cooperation with TP53 potentiates transcriptional activation of BAX promoter activity increasing neuronal cell apoptosis. Negatively regulates BAX promoter activity in the absence of TP53. Acts as a transcriptional coactivator via its interaction with the transcription factor ESR1 by enhancing its effect on estrogen response element (ERE)-containing promoter. Antagonizes the transcriptional stimulatory activity of POU4F1 by preventing its binding to an octamer motif. Involved in TNFSF11-mediated terminal osteoclast differentiation (By similarity).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-POU4F2 antibody

Nucleus; Nucleus speckle; Cytoplasm; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 290457652
• NCBI GeneID: 5458
• UniProt Accession #: Q12837; Q13883; Q14987; B1PJR6; B2RC84
• Molecular Weight: 409
• NCBI Official Full Name: POU domain, class 4, transcription factor 2
• NCBI Official Synonym Full Names: POU class 4 homeobox 2
• NCBI Official Symbol: POU4F2
• NCBI Official Synonym Symbols: BRN3B; BRN3.2; Brn-3b
• NCBI Protein Information: POU domain, class 4, transcription factor 2; brain-3B; POU domain protein; Brn3b POU domain transcription factor; POU domain class 4 transcription factor 2; brain-specific homeobox/POU domain protein 3B
• UniProt Protein Name: POU domain, class 4, transcription factor 2
• Protein Family: POU domain, class 4, transcription factor
• UniProt Gene Name: POU4F2
• UniProt Synonym Gene Names: BRN3B; Brain-3B; Brn-3B
• UniProt Entry Name: PO4F2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the POU-domain transcription factor family and may be involved in maintaining visual system neurons in the retina. The level of the encoded protein is also elevated in a majority of breast cancers, resulting in accelerated tumor growth. [provided by RefSeq, Sep 2011]

Uniprot Description

POU4F2: Transcription factor. May play a role in determining or maintaining the identities of a small subset of visual system neurons. Belongs to the POU transcription factor family. Class- 4 subfamily.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 4q31.2

Cellular Component: nuclear speck; nucleus

Molecular Function: chromatin binding

Biological Process: neuron differentiation; axon guidance; estrogen receptor signaling pathway; sensory perception of sound; transcription, DNA-dependent; retina development in camera-type eye; axon extension; MAPKKK cascade; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; neuromuscular process controlling balance; positive regulation of cell differentiation; retinal ganglion cell axon guidance