Rabbit anti-Human JHDM2a Polyclonal Antibody | anti-JHDM2a antibody

JHDM2a, CT (KDM3A, JHDM2A, JMJD1, JMJD1A, KIAA0742, TSGA, Lysine-specific demethylase 3A, JmjC domain-containing histone demethylation protein 2A, Jumonji domain-containing protein 1A) (FITC)

Cat. Num. AAA6311601

• Gene Names: KDM3A; TSGA; JMJD1; JHDM2A; JHMD2A; JMJD1A
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: JHDM2a; Polyclonal Antibody; CT (KDM3A; JHDM2A; JMJD1; JMJD1A; KIAA0742; TSGA; Lysine-specific demethylase 3A; JmjC domain-containing histone demethylation protein 2A; Jumonji domain-containing protein 1A) (FITC); KDM3A; Jumonji domain-containing protein 1A; anti-JHDM2a antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-JHDM2a antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: JHDM2a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 983-1017 amino acids from the C-terminal region of human JHDM2a.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(JHDM2a Antibody (C-term) western blot analysis in uterus tumor cell line lysates (35ug/lane).This demonstrates the JHDM2a antibody detected the JHDM2a protein (arrow).)

Immunohistochemistry (IHC)

(JHDM2a Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of JHDM2a Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-JHDM2a antibody

KDM3A is a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation.

Product Categories/Family for anti-JHDM2a antibody

Antibodies; Enzymes; Demethylase

References

Suikki, H.E., et al. Prostate 70(8):889-898(2010). Aston, K.I., et al. Hum. Reprod. 25(6):1383-1397(2010). Chen, H., et al. J. Biol. Chem. 285(10):7374-7383(2010). Krieg, A.J., et al. Mol. Cell. Biol. 30(1):344-353(2010). Sar, A., et al. Cell Tissue Res. 337(2):223-234(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 226442779
• NCBI GeneID: 55818
• NCBI Accession #: NP_001140160.1; NP_060903.2
• NCBI GenBank Nucleotide #: NM_001146688.1
• UniProt Accession #: Q9Y4C1; Q53S72; Q68D47; Q68UT9; Q6N050; Q8IY08; D6W5M3
• Molecular Weight: 147,341 Da
• NCBI Official Full Name: lysine-specific demethylase 3A
• NCBI Official Synonym Full Names: lysine (K)-specific demethylase 3A
• NCBI Official Symbol: KDM3A
• NCBI Official Synonym Symbols: TSGA; JMJD1; JHDM2A; JHMD2A; JMJD1A
• NCBI Protein Information: lysine-specific demethylase 3A
• UniProt Protein Name: Lysine-specific demethylase 3A
• UniProt Gene Name: KDM3A
• UniProt Synonym Gene Names: JHDM2A; JMJD1; JMJD1A; KIAA0742; TSGA
• UniProt Entry Name: KDM3A_HUMAN

NCBI Description

This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]

Uniprot Description

JMJD1A: Histone demethylase that specifically demethylates 'Lys- 9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. Belongs to the JHDM2 histone demethylase family.

Protein type: EC 1.14.11.-; Demethylase; Oxidoreductase; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 2p11.2

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus

Molecular Function: androgen receptor binding; dioxygenase activity; iron ion binding; transcription factor activity

Biological Process: negative regulation of histone H3-K9 methylation; formaldehyde biosynthetic process; establishment and/or maintenance of chromatin architecture; spermatid nuclear elongation; histone H3-K9 demethylation; hormone-mediated signaling; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter