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Western Blot (WB) (Western BlotPositive WB detected in: K562 whole cell lysate, COLO320 whole cell lysate, SH-SY5Y whole cell lysate, A549 whole cell lysate, PC3 whole cell lysateAll lanes: BRINP3 antibody at 3.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 89, 77 KDaObserved band size: 77 KDa)

Rabbit anti-Human BRINP3 Polyclonal Antibody | anti-BRINP3 antibody

BRINP3 Antibody

Gene Names
BRINP3; FAM5C; DBCCR1L; DBCCR1L1
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
>95%, Protein G purified
Synonyms
BRINP3; Polyclonal Antibody; BRINP3 Antibody; BMP/retinoic acid-inducible neural-specific protein 3; DBCCR1-like protein 1; DBCCR1L; DBCCR1L1; FAM5C; anti-BRINP3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
664
Applicable Applications for anti-BRINP3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:5000
IHC: 1:500-1:1000
Immunogen
Recombinant Human BMP/retinoic acid-inducible neural-specific protein 3 protein (389-487AA)
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: K562 whole cell lysate, COLO320 whole cell lysate, SH-SY5Y whole cell lysate, A549 whole cell lysate, PC3 whole cell lysateAll lanes: BRINP3 antibody at 3.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 89, 77 KDaObserved band size: 77 KDa)

Western Blot (WB) (Western BlotPositive WB detected in: K562 whole cell lysate, COLO320 whole cell lysate, SH-SY5Y whole cell lysate, A549 whole cell lysate, PC3 whole cell lysateAll lanes: BRINP3 antibody at 3.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 89, 77 KDaObserved band size: 77 KDa)

Immunohistochemistry (IHC)

(IHC image of MBS7110271 diluted at 1:600 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC) (IHC image of MBS7110271 diluted at 1:600 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Related Product Information for anti-BRINP3 antibody
Inhibits neuronal cell proliferation by negative regulation of the cell cycle transition. Promotes pituitary gonadotrope cell proliferation, migration and invasion, when overexpressed. May play a role in cell pituitary tumor development.
Product Categories/Family for anti-BRINP3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
76,299 Da
NCBI Official Full Name
BMP/retinoic acid-inducible neural-specific protein 3 isoform 2
NCBI Official Synonym Full Names
BMP/retinoic acid inducible neural specific 3
NCBI Official Symbol
BRINP3
NCBI Official Synonym Symbols
FAM5C; DBCCR1L; DBCCR1L1
NCBI Protein Information
BMP/retinoic acid-inducible neural-specific protein 3
UniProt Protein Name
BMP/retinoic acid-inducible neural-specific protein 3
UniProt Gene Name
BRINP3
UniProt Synonym Gene Names
DBCCR1L; DBCCR1L1; FAM5C

NCBI Description

This gene is overexpressed in pituitary tumors but is underexpressed in tongue squamous cell carcinomas, ulcerative colitis, and peri-implantitis. Polymorphisms that increase expression of this gene have been shown to increase vascular inflammation, and an association of this gene with myocardial infarction has been demonstrated. Finally, hypermethylation of this gene may find usefulness as a biomarker for gastric cancer. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

Uniprot Description

Inhibits neuronal cell proliferation by negative regulation of the cell cycle transition. Promotes pituitary gonadotrope cell proliferation, migration and invasion, when overexpressed. May play a role in cell pituitary tumor development.

Research Articles on BRINP3

Similar Products

Product Notes

The BRINP3 brinp3 (Catalog #AAA7110271) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BRINP3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BRINP3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:5000 IHC: 1:500-1:1000. Researchers should empirically determine the suitability of the BRINP3 brinp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRINP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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