Rabbit anti-Mouse BRD7 Polyclonal Antibody | anti-BRD7 antibody

BRD7, NT (BRD7, BP75, CELTIX1, Bromodomain-containing protein 7, 75kD bromodomain protein, Protein CELTIX-1) (Azide free) (HRP)

Cat. Num. AAA6278850

• Gene Names: BRD7; BP75; NAG4; CELTIX1
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BRD7; Polyclonal Antibody; NT (BRD7; BP75; CELTIX1; Bromodomain-containing protein 7; 75kD bromodomain protein; Protein CELTIX-1) (Azide free) (HRP); Protein CELTIX-1; anti-BRD7 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-BRD7 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: BRD7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-107 amino acids from the N-terminal region of human BRD7.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(BRD7 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the BRD7 antibody detected the BRD7 protein (arrow).)

Related Product Information for anti-BRD7 antibody

This gene encodes a protein which is a member of the bromodomain-containing protein family. The product of this gene has been identified as a component of one form of the SWI/SNF chromatin remodeling complex, and as a protein which interacts with p53 and is required for p53-dependent oncogene-induced senescence which prevents tumor growth. Pseudogenes have been described on chromosomes 2, 3, 6, 13 and 14. Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-BRD7 antibody

Antibodies; Proteins

References

Burrows, A.E., et al. Proc. Natl. Acad. Sci. U.S.A. 107(32):14280-14285(2010). Drost, J., et al. Nat. Cell Biol. 12(4):380-389(2010). Kikuchi, M., et al. Biochim. Biophys. Acta 1793(12):1828-1836(2009). Kaeser, M.D., et al. J. Biol. Chem. 283(47):32254-32263(2008). Liu, H., et al. BMC Mol. Biol. 9, 111 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 291219913
• NCBI GeneID: 29117
• NCBI Accession #: NP_001167455.1; NP_037395.2
• NCBI GenBank Nucleotide #: NM_001173984.2
• UniProt Accession #: Q9NPI1; Q4VC09; Q8N2L9; Q96KA4; Q9BV48; Q9UH59
• Molecular Weight: 74,139 Da
• NCBI Official Full Name: bromodomain-containing protein 7 isoform 1
• NCBI Official Synonym Full Names: bromodomain containing 7
• NCBI Official Symbol: BRD7
• NCBI Official Synonym Symbols: BP75; NAG4; CELTIX1
• NCBI Protein Information: bromodomain-containing protein 7; protein CELTIX-1; 75 kDa bromodomain protein
• UniProt Protein Name: Bromodomain-containing protein 7
• Protein Family: Bromodomain-containing protein
• UniProt Gene Name: BRD7
• UniProt Synonym Gene Names: BP75; CELTIX1
• UniProt Entry Name: BRD7_HUMAN

NCBI Description

This gene encodes a protein which is a member of the bromodomain-containing protein family. The product of this gene has been identified as a component of one form of the SWI/SNF chromatin remodeling complex, and as a protein which interacts with p53 and is required for p53-dependent oncogene-induced senescence which prevents tumor growth. Pseudogenes have been described on chromosomes 2, 3, 6, 13 and 14. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]

Uniprot Description

BRD7: a bromodomain protein that binds dishevelled-1 (DVL1) and enhances Wnt signaling by inhibiting GSK3B. Interacts with DVL1, PTPN13, IRF2 and HNRPUL1. Associates with histones and E1B-AP5. Binds acetylated histone peptides, most notably H3 peptide acetylated at Lys14. Associates with histones and E1B-AP5. Its bromodomain is found in many chromatin-associated proteins and most known histone acetyltransferases. Inhibits G1-S progression by transcriptional regulation of molecules in the ras and Rb pathways. Plays an important role in transcriptional activation and chromatin remodeling. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription regulation; Tumor suppressor; Cell cycle regulation; Histone-binding

Chromosomal Location of Human Ortholog: 16q12

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; histone binding; p53 binding; transcription coactivator activity; transcription factor binding; transcription corepressor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; Wnt receptor signaling pathway; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of histone acetylation; negative regulation of transcription, DNA-dependent; cell cycle