Rabbit anti-Mouse BRD7 Polyclonal Antibody | anti-BRD7 antibody

BRD7, NT (BRD7, BP75, CELTIX1, Bromodomain-containing protein 7, 75kD bromodomain protein, Protein CELTIX-1)

Cat. Num. AAA647197

• Gene Names: BRD7; BP75; NAG4; CELTIX1
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: BRD7; Polyclonal Antibody; NT (BRD7; BP75; CELTIX1; Bromodomain-containing protein 7; 75kD bromodomain protein; Protein CELTIX-1); Anti -BRD7; anti-BRD7 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-BRD7 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: BRD7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-107 amino acids from the N-terminal region of human BRD7.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(BRD7 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the BRD7 antibody detected the BRD7 protein (arrow).)

Related Product Information for anti-BRD7 antibody

This gene encodes a protein which is a member of the bromodomain-containing protein family. The product of this gene has been identified as a component of one form of the SWI/SNF chromatin remodeling complex, and as a protein which interacts with p53 and is required for p53-dependent oncogene-induced senescence which prevents tumor growth. Pseudogenes have been described on chromosomes 2, 3, 6, 13 and 14. Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-BRD7 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 291219913
• NCBI GeneID: 29117
• NCBI Accession #: NP_001167455.1
• NCBI GenBank Nucleotide #: NM_001173984.2
• UniProt Accession #: Q9NPI1; Q4VC09; Q8N2L9; Q96KA4; Q9BV48; Q9UH59
• Molecular Weight: 74,139 Da
• NCBI Official Full Name: bromodomain-containing protein 7 isoform 1
• NCBI Official Synonym Full Names: bromodomain containing 7
• NCBI Official Symbol: BRD7
• NCBI Official Synonym Symbols: BP75; NAG4; CELTIX1
• NCBI Protein Information: bromodomain-containing protein 7; protein CELTIX-1; 75 kDa bromodomain protein
• UniProt Protein Name: Bromodomain-containing protein 7
• Protein Family: Bromodomain-containing protein
• UniProt Gene Name: BRD7
• UniProt Synonym Gene Names: BP75; CELTIX1
• UniProt Entry Name: BRD7_HUMAN

NCBI Description

This gene encodes a protein which is a member of the bromodomain-containing protein family. The product of this gene has been identified as a component of one form of the SWI/SNF chromatin remodeling complex, and as a protein which interacts with p53 and is required for p53-dependent oncogene-induced senescence which prevents tumor growth. Pseudogenes have been described on chromosomes 2, 3, 6, 13 and 14. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]

Uniprot Description

Function: Acts both as coactivator and as corepressor. May play a role in chromatin remodeling. Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at 'Tyr-216'. Down-regulates TRIM24-mediated activation of transcriptional activation by AR

By similarity. Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at 'Lys-9' (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at 'Lys-382', and thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase. Ref.8 Ref.9 Ref.11 Ref.12 Ref.13

Subunit structure: Interacts with TRIM24, PTPN13 and DVL1. Identified in a complex with SMARCA4/BRG1, SMARCC1/BAF155, SMARCE1/BAF57, DPF2/BAF45D and ARID2, subunits of the SWI/SNF-B (PBAF) chromatin remodeling complex

By similarity. Interacts with IRF2 and HNRPUL1. Interacts (via N-terminus) with TP53. Interacts (via C-terminus) with EP300. Interacts with BRCA1. Interacts (via bromo domain) with histone H3 (via N-terminus) acetylated at 'Lys-14' (H3K14ac). Has low affinity for histone H3 acetylated at 'Lys-9' (H3K9ac). Has the highest affinity for histone H3 that is acetylated both at 'Lys-9' (H3K9ac) and at 'Lys-14' (H3K14ac). Has very low affinity for non-acetylated histone H3. Interacts (via bromo domain) with histone H4 (via N-terminus) acetylated at 'Lys-8' (H3K8ac) (in vitro). Ref.1 Ref.2 Ref.8 Ref.11 Ref.12 Ref.13 Ref.16

Subcellular location: Isoform 2: Nucleus Ref.1 Ref.8 Ref.9 Ref.12.

Sequence similarities: Contains 1 bromo domain.

Sequence caution: The sequence AAH01611.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence BAB55031.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence BAC11089.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.