Rabbit anti-Mouse BRD7 Polyclonal Antibody | anti-BRD7 antibody

BRD7, NT (BRD7, BP75, CELTIX1, Bromodomain-containing protein 7, 75kD bromodomain protein, Protein CELTIX-1) (FITC)

Cat. Num. AAA6278849

• Gene Names: BRD7; BP75; NAG4; CELTIX1
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BRD7; Polyclonal Antibody; NT (BRD7; BP75; CELTIX1; Bromodomain-containing protein 7; 75kD bromodomain protein; Protein CELTIX-1) (FITC); Protein CELTIX-1; anti-BRD7 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-BRD7 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: BRD7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-107 amino acids from the N-terminal region of human BRD7.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(BRD7 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the BRD7 antibody detected the BRD7 protein (arrow).)

Related Product Information for anti-BRD7 antibody

This gene encodes a protein which is a member of the bromodomain-containing protein family. The product of this gene has been identified as a component of one form of the SWI/SNF chromatin remodeling complex, and as a protein which interacts with p53 and is required for p53-dependent oncogene-induced senescence which prevents tumor growth. Pseudogenes have been described on chromosomes 2, 3, 6, 13 and 14. Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-BRD7 antibody

Antibodies; Proteins

References

Burrows, A.E., et al. Proc. Natl. Acad. Sci. U.S.A. 107(32):14280-14285(2010). Drost, J., et al. Nat. Cell Biol. 12(4):380-389(2010). Kikuchi, M., et al. Biochim. Biophys. Acta 1793(12):1828-1836(2009). Kaeser, M.D., et al. J. Biol. Chem. 283(47):32254-32263(2008). Liu, H., et al. BMC Mol. Biol. 9, 111 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 291219913
• NCBI GeneID: 29117
• NCBI Accession #: NP_001167455.1; NP_037395.2
• NCBI GenBank Nucleotide #: NM_001173984.2
• UniProt Accession #: Q9NPI1; Q4VC09; Q8N2L9; Q96KA4; Q9BV48; Q9UH59
• Molecular Weight: 74,139 Da
• NCBI Official Full Name: bromodomain-containing protein 7 isoform 1
• NCBI Official Synonym Full Names: bromodomain containing 7
• NCBI Official Symbol: BRD7
• NCBI Official Synonym Symbols: BP75; NAG4; CELTIX1
• NCBI Protein Information: bromodomain-containing protein 7; protein CELTIX-1; 75 kDa bromodomain protein
• UniProt Protein Name: Bromodomain-containing protein 7
• Protein Family: Bromodomain-containing protein
• UniProt Gene Name: BRD7
• UniProt Synonym Gene Names: BP75; CELTIX1
• UniProt Entry Name: BRD7_HUMAN

NCBI Description

This gene encodes a protein which is a member of the bromodomain-containing protein family. The product of this gene has been identified as a component of one form of the SWI/SNF chromatin remodeling complex, and as a protein which interacts with p53 and is required for p53-dependent oncogene-induced senescence which prevents tumor growth. Pseudogenes have been described on chromosomes 2, 3, 6, 13 and 14. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]

Uniprot Description

BRD7: a bromodomain protein that binds dishevelled-1 (DVL1) and enhances Wnt signaling by inhibiting GSK3B. Interacts with DVL1, PTPN13, IRF2 and HNRPUL1. Associates with histones and E1B-AP5. Binds acetylated histone peptides, most notably H3 peptide acetylated at Lys14. Associates with histones and E1B-AP5. Its bromodomain is found in many chromatin-associated proteins and most known histone acetyltransferases. Inhibits G1-S progression by transcriptional regulation of molecules in the ras and Rb pathways. Plays an important role in transcriptional activation and chromatin remodeling. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription regulation; Tumor suppressor; Cell cycle regulation; Histone-binding

Chromosomal Location of Human Ortholog: 16q12

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; histone binding; p53 binding; transcription coactivator activity; transcription factor binding; transcription corepressor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; Wnt receptor signaling pathway; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of histone acetylation; negative regulation of transcription, DNA-dependent; cell cycle