Rabbit anti-Human BRD4 Polyclonal Antibody | anti-BRD4 antibody

BRD4, NT (Bromodomain-containing Protein 4, Protein HUNK1, HUNK1)

Cat. Num. AAA628489

• Gene Names: BRD4; CAP; MCAP; HUNK1; HUNKI
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: BRD4; Polyclonal Antibody; NT (Bromodomain-containing Protein 4; Protein HUNK1; HUNK1); Anti -BRD4; anti-BRD4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BRD4.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-BRD4 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the N-terminal region of human BRD4 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Immunohistochemistry of formalin-fixed and paraffin-embedded human cancer tissue using MBS628489, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of MBS628489 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-BRD4 antibody

BRD4 is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. The gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people.

Product Categories/Family for anti-BRD4 antibody

Antibodies; Abs to Mitosis Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 116283901
• NCBI GeneID: 23476
• UniProt Accession #: Q6NXE4; O60885; Q05BM2; Q4G0X8; Q5BJ26; Q6PIS5; Q96HN0
• Molecular Weight: 60,885 Da
• NCBI Official Full Name: BRD4 protein
• NCBI Official Synonym Full Names: bromodomain containing 4
• NCBI Official Symbol: BRD4
• NCBI Official Synonym Symbols: CAP; MCAP; HUNK1; HUNKI
• NCBI Protein Information: bromodomain-containing protein 4; bromodomain-containing 4; chromosome-associated protein
• UniProt Protein Name: BRD4 protein
• Protein Family: Bromodomain-containing protein
• UniProt Gene Name: BRD4
• UniProt Entry Name: Q6NXE4_HUMAN

NCBI Description

The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq]

Uniprot Description

BRD4: an atypical protein kinase of the Brd family. Contains a double bromodomain. Binds to chromatin and regulates cell cycle progression at multiple stages. Associates with chromosomes during mitosis. The cognate gene is the chromosome 19 target of translocation t(15;19)(q13;p13.1), resulting in the BRD4-NUT oncogene identified in a lethal carcinoma of young people. Binds to papillomavirus E2 protein and is required for its transforming ability. Two alternatively spliced isoforms have been described.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, atypical; ATYPICAL group; BRD family

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: nucleoplasm; transcription elongation factor complex b; condensed nuclear chromosome; cytoplasm; nuclear chromatin; chromosome; nucleus

Molecular Function: protein binding; DNA binding; p53 binding; chromatin binding

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; transcription, DNA-dependent; protein amino acid phosphorylation; inner cell mass cell proliferation; chromosome segregation; chromatin remodeling; G1/S-specific transcription in mitotic cell cycle; positive regulation of RNA elongation from RNA polymerase II promoter; regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter; response to DNA damage stimulus; positive regulation of DNA binding