Rabbit anti-Human, Mouse CBX8 Polyclonal Antibody | anti-CBX8 antibody

CBX8, CT (Chromobox Protein Homolog 8, Polycomb 3 Homolog, Pc3, HPC3, Rectachrome 1, PC3, RC1) (PE)

Cat. Num. AAA6467817

• Gene Names: CBX8; PC3; RC1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: CBX8; Polyclonal Antibody; CT (Chromobox Protein Homolog 8; Polycomb 3 Homolog; Pc3; HPC3; Rectachrome 1; PC3; RC1) (PE); anti-CBX8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CBX8. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CBX8 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human CBX8 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(The anti-CBX8 C-term Pab is used in Western blot to detect CBX8 in mouse kidney tissue lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(CBX8 Antibody (Y321) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CBX8 antibody detected the CBX8 protein (arrow).)

Product Categories/Family for anti-CBX8 antibody

Antibodies; Chromatin Proteins

References

1. Ota, T., et al., Nat. Genet. 36(1):40-45 (2004). 2. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002). 3. Bardos, J.I., et al., J. Biol. Chem. 275(37):28785-28792 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 78099843
• NCBI GeneID: 57332
• UniProt Accession #: Q9HC52; Q96H39; Q9NR07
• Molecular Weight: 389
• NCBI Official Full Name: Chromobox protein homolog 8
• NCBI Official Synonym Full Names: chromobox homolog 8
• NCBI Official Symbol: CBX8
• NCBI Official Synonym Symbols: PC3; RC1
• NCBI Protein Information: chromobox protein homolog 8; polycomb 3; rectachrome 1; Pc class 3 homolog; chromobox homolog 8 (Pc class homolog, Drosophila)
• UniProt Protein Name: Chromobox protein homolog 8
• Protein Family: Chromobox protein
• UniProt Gene Name: CBX8
• UniProt Synonym Gene Names: PC3; RC1; Pc3; hPc3
• UniProt Entry Name: CBX8_HUMAN

Uniprot Description

CBX8: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: nucleoplasm; heterochromatin; nuclear chromatin; PcG protein complex; nucleus

Molecular Function: protein binding; single-stranded RNA binding; ubiquitin-protein ligase activity; methylated histone residue binding

Biological Process: transcription, DNA-dependent; histone ubiquitination; negative regulation of transcription from RNA polymerase II promoter