Rabbit anti-Human BRD4 Polyclonal Antibody | anti-BRD4 antibody

BRD4, CT (BRD4-NUT fusion oncoprotein, BRD4-NUT FUSION) (APC)

Cat. Num. AAA6278825

• Gene Names: BRD4; CAP; MCAP; HUNK1; HUNKI
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: BRD4; Polyclonal Antibody; CT (BRD4-NUT fusion oncoprotein; BRD4-NUT FUSION) (APC); BRD4-NUT fusion oncoprotein; BRD4-NUT FUSION; anti-BRD4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BRD4.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-BRD4 antibody: Immunohistochemistry (IHC), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1313-1342 in the C-terminal region of human BRD4, UniProt Accession #O60885. Species sequence homology: mouse
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue using MBS6008792 followed by peroxidase-conjugated secondary antibody, followed by AEC staining. This data demonstrates the use of MBS6008792 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Testing Data

(Subnuclear distribution of cellular proteins. CHOBgl40 cells grown on coverslips were either directly or after treatment with 0.5% Triton X-100, incubated with MBS6008792 (images 4 and 5). PI, propidium iodide staining of cellular DNA (images 6).WC, whole cells.)

Related Product Information for anti-BRD4 antibody

BRD4 is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. The gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people.

Product Categories/Family for anti-BRD4 antibody

Antibodies; Oncoproteins

References

1. Maruyama, T., et al., Mol. Cell. Biol. 22(18):6509-6520 (2002). 2. French, C.A., et al., Am. J. Pathol. 159(6):1987-1992 (2001). 3. Dey, A., et al., Mol. Cell. Biol. 20(17):6537-6549 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 7657218
• NCBI GeneID: 23476
• NCBI Accession #: NP_055114.1
• NCBI GenBank Nucleotide #: NM_014299.2
• UniProt Accession #: O60885; O60433; Q4G0X8; Q86YS8; Q96PD3
• Molecular Weight: 152kDa
• NCBI Official Full Name: bromodomain-containing protein 4 isoform short
• NCBI Official Synonym Full Names: bromodomain containing 4
• NCBI Official Symbol: BRD4
• NCBI Official Synonym Symbols: CAP; MCAP; HUNK1; HUNKI
• NCBI Protein Information: bromodomain-containing protein 4; bromodomain-containing 4; chromosome-associated protein
• UniProt Protein Name: Bromodomain-containing protein 4
• Protein Family: Bromodomain-containing protein
• UniProt Gene Name: BRD4
• UniProt Synonym Gene Names: HUNK1
• UniProt Entry Name: BRD4_HUMAN