Western Blot (WB)
(Western blot analysis of BOB1 expression in HT29 (A), mouse spleen (B), rat thymus (C) whole cell lysates.)
Rabbit anti-Human BOB1 Polyclonal Antibody | anti-POU2AF1 antibody
Anti-BOB1 Antibody
Western Blot (WB)
(Western blot analysis of BOB1 expression in HT29 (A), mouse spleen (B), rat thymus (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of BOB1 expression in HT29 (A), mouse spleen (B), rat thymus (C) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of BOB1 staining in HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of BOB1 staining in HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
Uniprot Description
POU2AF1: Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6. Belongs to the POU2AF1 family.
Protein type: Transcription, coactivator/corepressor; Oncoprotein
Chromosomal Location of Human Ortholog: 11q23.1
Molecular Function: protein binding; transcription coactivator activity; transcription cofactor activity
Biological Process: humoral immune response; transcription from RNA polymerase II promoter
Research Articles on POU2AF1
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Product Notes
The POU2AF1 pou2af1 (Catalog #AAA8247587) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-BOB1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BOB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). Western Blot: 1/500 - 1/2000, Immunofluorescence/Immunocytochemistry: 1/50 - 1/200. Researchers should empirically determine the suitability of the POU2AF1 pou2af1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BOB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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