Anoctamin 1 Recombinant Protein | ANO1 recombinant protein

Recombinant Anoctamin 1 (ANO1)

Cat. Num. AAA2029536

• Gene Names: ANO1; DOG1; TAOS2; ORAOV2; TMEM16A
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Anoctamin 1; Recombinant Anoctamin 1 (ANO1); ANO1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 6 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- S ADRECQRGPE TIAHEAQDAG TPNSGDATGV VDGEREATMR VPEKYSTLPA EDRSVHIVNI CAIEDLGYLP SEGTLLNSLS VDPDAECKYG LYFRDGKRKV DYILVYHHKR ASGSRTLARR GLQNDMVLGT RSVRQDQPLP GKGSPVDAGS PEVPMDYHED DKRFRREEYE GNLLEAGLEL ENDEDTKIHG VGFVKIHAPWHVLCREAEFL KLKMPTKKV
• Sequence Length: 986

• Applicable Applications for ANO1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 28.4kDa
• Accurate Molecular Mass (KD): 38kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Ser20~Val239
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 194306539
• NCBI GeneID: 55107
• NCBI Accession #: NP_060513.5
• NCBI GenBank Nucleotide #: NM_018043.5
• Molecular Weight: 74,369 Da
• NCBI Official Full Name: anoctamin-1
• NCBI Official Synonym Full Names: anoctamin 1, calcium activated chloride channel
• NCBI Official Symbol: ANO1
• NCBI Official Synonym Symbols: DOG1; TAOS2; ORAOV2; TMEM16A
• NCBI Protein Information: anoctamin-1
• UniProt Protein Name: Anoctamin-1
• Protein Family: Anoctamin
• UniProt Gene Name: ANO1
• UniProt Synonym Gene Names: DOG1; ORAOV2; TAOS2; TMEM16A
• UniProt Entry Name: ANO1_HUMAN

Uniprot Description

ANO1: Acts as a calcium-activated chloride channel. Required for normal tracheal development. Belongs to the anoctamin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Membrane protein, integral; Membrane protein, multi-pass; Transporter, ion channel

Chromosomal Location of Human Ortholog: 11q13.3

Cellular Component: apical plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; protein homodimerization activity; intracellular calcium activated chloride channel activity; protein heterodimerization activity; calcium activated cation channel activity

Biological Process: regulation of membrane potential; multicellular organismal development; chloride transport; transmembrane transport; cation transport