Rabbit anti-Human ZNF324B Polyclonal Antibody | anti-ZNF324B antibody

ZNF324B, CT (ZNF324B, Zinc finger protein 324B) (Azide free) (HRP)

Cat. Num. AAA6366243

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ZNF324B; Polyclonal Antibody; CT (ZNF324B; Zinc finger protein 324B) (Azide free) (HRP); Zinc finger protein 324B; anti-ZNF324B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ZNF324B antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ZNF324B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 514-543 amino acids from the C-terminal region of human ZNF324B.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ZNF324B Antibody (C-term) western blot analysis in CEM and K562 cell line lysates (35ug/lane).This demonstrates the ZNF324B antibody detected the ZNF324B protein (arrow).)

Immunohistochemistry (IHC)

(ZNF324B antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZNF324B antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ZNF324B Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ZNF324B antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

Ota, T., et al. Nat. Genet. 36(1):40-45(2004). Strausberg, R.L., et al. Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002). Bonaldo, M.F., et al. Genome Res. 6(9):791-806(1996).

NCBI and Uniprot Product Information

• NCBI GI #: 194018495
• NCBI GeneID: 388569
• NCBI Accession #: NP_997278.2
• NCBI GenBank Nucleotide #: NM_207395.2
• UniProt Accession #: Q6AW86; Q6ZMX8; Q6ZS42; B2RTZ6
• Molecular Weight: 60,602 Da
• NCBI Official Full Name: zinc finger protein 324B
• NCBI Official Synonym Full Names: zinc finger protein 324B
• NCBI Official Symbol: ZNF324B
• NCBI Protein Information: zinc finger protein 324B
• UniProt Protein Name: Zinc finger protein 324B
• Protein Family: Zinc finger protein
• UniProt Gene Name: ZNF324B
• UniProt Entry Name: Z324B_HUMAN