Typical Testing Data/Standard Curve (for reference only)
Rat Interleukin 1 receptor antagonist ELISA Kit | IL-1ra/CD121 elisa kit
Rat Interleukin 1 receptor antagonist ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: IL-1RA ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IL-1RA antibody and an IL-1RA-HRP conjugate. The assay sample and buffer are incubated together with IL-1RA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-1RA concentration since IL-1RA from samples and IL-1RA-HRP conjugate compete for the anti-IL-1RA antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-1RA from the sample, fewer sites are left to bind IL-1RA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-1RA concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA). It is an important mediator involved in many cytokine induced immune and inflammatory responses. This gene along with interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 2 (IL1RL2), and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. [provided by RefSeq, Jul 2008]
Uniprot Description
IL-1RA: Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the corecptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. Belongs to the interleukin-1 receptor family.
Protein type: EC 2.7.11.25; Membrane protein, integral; Receptor, cytokine
Chromosomal Location of Human Ortholog: 2q12
Cellular Component: cell surface; membrane; integral to plasma membrane; plasma membrane; extracellular region
Molecular Function: interleukin-1, Type I, activating receptor activity; protein binding; signal transducer activity; transmembrane receptor activity; protease binding; platelet-derived growth factor receptor binding; interleukin-1 receptor activity
Biological Process: cell surface receptor linked signal transduction; regulation of inflammatory response; immune response
Research Articles on IL-1ra/CD121
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Product Notes
The Rat IL-1ra/CD121 il1r1 (Catalog #AAA730139) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA730139 ELISA Kit recognizes Rat IL-1ra/CD121. It is sometimes possible for the material contained within the vial of "Interleukin 1 receptor antagonist, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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