Rabbit BNIP-2 Polyclonal Antibody | anti-BNIP-2 antibody

BNIP-2 Polyclonal Antibody

Cat. Num. AAA3005711

• Gene Names: BNIP2; NIP2; BNIP-2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: BNIP-2; Polyclonal Antibody; BNIP-2 Polyclonal Antibody; BCL2/adenovirus E1B 19 kDa protein-interacting protein 2; BNIP2; BNIP 2; NIP2; anti-BNIP-2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: BNIP-2 polyclonal antibody detects endogenous levels of BNIP-2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 435

• Applicable Applications for anti-BNIP-2 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 269-314 of Human BNIP-2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of BNIP-2 polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:Raw264. 7 cell lysateLane3:PC12 cell lysate)

Related Product Information for anti-BNIP-2 antibody

BNIP-2, (previously designated Nip2 and Nip21 in human and mouse respectively), shares homology with the non-catalytic domain of Cdc42 GTPase-activating protein (Cdc42GAP). Through binding to Cdc42GAP, BNIP-2 enhances the GTPase activity of Cdc42GAP, facilitating the hydrolysis of GTP bound to Cdc42 and thereby, mediating the signaling pathways involving receptor kinases, small GTPases and apoptotic proteins. Nix, which is also designated Nip3L or Bnip3L, is highly related to BNIP-3, and both proteins localize to the mitochondria where they associate with Bcl-2 proteins.

NCBI and Uniprot Product Information

• NCBI GI #: 168480080
• NCBI GeneID: 663
• NCBI Accession #: NP_004321.2
• NCBI GenBank Nucleotide #: NP_004321.2
• UniProt Accession #: Q12982
• Molecular Weight: ~ 36kDa
• NCBI Official Full Name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 2 isoform 2
• NCBI Official Synonym Full Names: BCL2 interacting protein 2
• NCBI Official Symbol: BNIP2
• NCBI Official Synonym Symbols: NIP2; BNIP-2
• NCBI Protein Information: BCL2/adenovirus E1B 19 kDa protein-interacting protein 2
• UniProt Protein Name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 2
• UniProt Gene Name: BNIP2
• UniProt Synonym Gene Names: NIP2
• UniProt Entry Name: BNIP2_HUMAN

NCBI Description

This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death. The encoded protein also interacts with E1B 19 kDa-like sequences of BCL2, another apoptotic protector. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Uniprot Description

BNIP2: Implicated in the suppression of cell death. Interacts with the BCL-2 and adenovirus E1B 19 kDa proteins. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q22.2

Cellular Component: intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; nuclear envelope; cytosol

Molecular Function: identical protein binding; protein binding; calcium ion binding; GTPase activator activity

Biological Process: muscle cell differentiation; apoptosis; positive regulation of muscle cell differentiation; positive regulation of GTPase activity; negative regulation of apoptosis