Rabbit anti-Human, Mouse Mapkapk3 Polyclonal Antibody | anti-Mapkapk3 antibody

Mapkapk3, NT (Mapkapk3, MAP kinase-activated protein kinase 3) (Azide free) (HRP)

Cat. Num. AAA6318895

• Gene Names: Mapkapk3; 3PK; MK3; MK-3; MAPKAP3; AI874665; MAPKAP-K3; MapkKapk3
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Mapkapk3; Polyclonal Antibody; NT (Mapkapk3; MAP kinase-activated protein kinase 3) (Azide free) (HRP); MAP kinase-activated protein kinase 3; anti-Mapkapk3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-Mapkapk3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: Mouse Mapkapk3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-90 amino acids from the N-terminal region of Mouse Mapkapk3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Mapkapk3 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the Mapkapk3 antibody detected the Mapkapk3 protein (arrow).)

Western Blot (WB)

(Mouse Mapkapk3 Antibody (N-term) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the Mapkapk3 antibody detected the Mapkapk3 protein (arrow).)

Immunohistochemistry (IHC)

(Mouse Mapkapk3 Antibody (N-term) (AP14268a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Mapkapk3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-Mapkapk3 antibody

Modulator of polycomb-mediated repression, which can be activated either by ERK, p38 and JNK. Substrate of CSBP (By similarity).

Product Categories/Family for anti-Mapkapk3 antibody

Antibodies; Protein Kinases

References

Menon, M.B., et al. J. Biol. Chem. 285(43):33242-33251(2010). Moise, N., et al. Cell. Signal. 22(10):1502-1512(2010). Ronkina, N., et al. Mol. Cell. Biol. 27(1):170-181(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 31542089
• NCBI GeneID: 102626
• NCBI Accession #: NP_849238.1
• NCBI GenBank Nucleotide #: NM_178907.3
• UniProt Accession #: Q3UMW7; Q3T9E6; Q8K0G3; B0QZU7; E9QNE1
• Molecular Weight: 43,293 Da
• NCBI Official Full Name: MAP kinase-activated protein kinase 3
• NCBI Official Synonym Full Names: mitogen-activated protein kinase-activated protein kinase 3
• NCBI Official Symbol: Mapkapk3
• NCBI Official Synonym Symbols: 3PK; MK3; MK-3; MAPKAP3; AI874665; MAPKAP-K3; MapkKapk3
• NCBI Protein Information: MAP kinase-activated protein kinase 3; MAPKAPK-3; MAPKAP kinase 3; MAPK-activated protein kinase 3; mitogen activated protein kinase-activated protein kinase 3
• UniProt Protein Name: MAP kinase-activated protein kinase 3
• Protein Family: MAP kinase-activated protein kinase
• UniProt Gene Name: Mapkapk3
• UniProt Synonym Gene Names: MAPK-activated protein kinase 3; MAPKAP kinase 3; MAPKAP-K3; MAPKAPK-3; MK-3
• UniProt Entry Name: MAPK3_MOUSE