Western Blot (WB)
(Fig. Western Blot analysis of 4T1 cells using IKKalpha/beta Polyclonal Antibody diluted at 1:1000.)
Rabbit IKKalpha/beta Polyclonal Antibody | anti-IKKalpha/beta antibody
IKKalpha/beta Polyclonal Antibody
Gene Names
CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
IKKalpha/beta; Polyclonal Antibody; IKKalpha/beta Polyclonal Antibody; Rabbit Anti-IKKalpha/beta Polyclonal Antibody; CHUK; IKKA; TCF16; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B; anti-IKKalpha/beta antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
IKKalpha/beta Polyclonal Antibody detects endogenous levels of IKKalpha/beta protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid; PBS Containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
745
Applicable Applications for anti-IKKalpha/beta antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:100-1:300
ELISA: 1:20000
IHC-P: 1:100-1:300
ELISA: 1:20000
Immunogen
Synthesized peptide derived from human IKKalpha/beta around the non-phosphorylation site of S176/177
Preparation and Storage
Stable for one year at -20 degree C from date of shipment.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Western Blot (WB)
(Fig. Western Blot analysis of 4T1 cells using IKKalpha/beta Polyclonal Antibody diluted at 1:1000.)
Western Blot (WB)
(Fig. Western Blot analysis of 4T1 cells using IKKalpha/beta Polyclonal Antibody diluted at 1:1000.)
Related Product Information for anti-IKKalpha/beta antibody
CHUK encodes a member of the serine/threonine protein kinase family. The conserved helix-loop-helix ubiquitous kinase, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
inhibitor of nuclear factor kappa-B kinase subunit alpha isoform 1
NCBI Official Synonym Full Names
component of inhibitor of nuclear factor kappa B kinase complex
NCBI Official Symbol
CHUK
NCBI Official Synonym Symbols
IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
NCBI Protein Information
inhibitor of nuclear factor kappa-B kinase subunit alpha
UniProt Protein Name
Inhibitor of nuclear factor kappa-B kinase subunit alpha
UniProt Gene Name
CHUK
UniProt Synonym Gene Names
IKKA; TCF16; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; IKK1; NFKBIKA; TCF-16
UniProt Entry Name
IKKA_HUMAN
NCBI Description
This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008]
Research Articles on IKKalpha/beta
Similar Products
Product Notes
The IKKalpha/beta chuk (Catalog #AAA9704292) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IKKalpha/beta Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IKKalpha/beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA). WB: 1:500-1:2000 IHC-P: 1:100-1:300 ELISA: 1:20000. Researchers should empirically determine the suitability of the IKKalpha/beta chuk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IKKalpha/beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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