Rabbit BNIP3L Polyclonal Antibody | anti-BNIP3L antibody

BNIP3L Polyclonal Antibody

Cat. Num. AAA3011482

• Gene Names: BNIP3L; NIX; BNIP3a
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: BNIP3L; Polyclonal Antibody; BNIP3L Polyclonal Antibody; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like; Adenovirus E1B19K-binding protein B5; B CL2/adenovirus E1B 19 kDa protein-interacting protein 3A; NIP3-like protein X; NIP3L; BNIP3A; BNIP3H; NIX; anti-BNIP3L antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: BNIP3L polyclonal antibody detects endogenous levels of BNIP3L protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1337

• Applicable Applications for anti-BNIP3L antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant full length Human BNIP3L.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of BNIP3L polyclonal antibody)

Related Product Information for anti-BNIP3L antibody

The adenovirus E1B protein is a viral homolog of the Bcl-2 family of proteins that are involved in regulating cell death. A family of interacting proteins, which are designated Nip or Bnip and include Nip1, Nip2, Nip3 and Nix, associate with both the E1B protein and Bcl-2 proteins to mediate apoptotic signaling. Nip1 contains a hydrophobic transmembrane domain, which enables its localization to the nuclear envelope, endoplasmic recticulum and mitochondria. Nip2 shares homology with the non-catalytic domain of Cdc42 GTPase-activating protein (Cdc42GAP). Through binding to Cdc42GAP, Nip2 enhances the GTPase activity of Cdc42GAP, facilitating the hydrolysis of GTP bound to Cdc42 and thereby, mediating the signaling pathways involving receptor kinases, small GTPases and apoptotic proteins. Nix, which is also designated Nip3L or Bnip3L, is highly related to Nip3, and both proteins localize to the mitochondria where they associate with Bcl-2 proteins. Nip3 preferentially binds to Bcl-xL and induces apoptosis by suppressing the anti-apoptosis activity of Bcl-xL.

NCBI and Uniprot Product Information

• NCBI GI #: 4138825
• NCBI GeneID: 665
• UniProt Accession #: O60238
• Molecular Weight: ~ 30, 45kDa
• NCBI Official Full Name: Homo sapiens NIX (NIX) mRNA, nuclear gene encoding mitochondrial protein, complete cds
• NCBI Official Synonym Full Names: BCL2 interacting protein 3 like
• NCBI Official Symbol: BNIP3L
• NCBI Official Synonym Symbols: NIX; BNIP3a
• NCBI Protein Information: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
• UniProt Protein Name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
• Protein Family: BCL2/adenovirus E1B 19 kDa protein-interacting protein
• UniProt Gene Name: BNIP3L
• UniProt Synonym Gene Names: BNIP3A; BNIP3H; NIX; NIP3L
• UniProt Entry Name: BNI3L_HUMAN

NCBI Description

This gene encodes a protein that belongs to the pro-apoptotic subfamily within the Bcl-2 family of proteins. The encoded protein binds to Bcl-2 and possesses the BH3 domain. The protein directly targets mitochondria and causes apoptotic changes, including loss of membrane potential and the release of cytochrome c. [provided by RefSeq, Feb 2015]

Uniprot Description

BNIP3L: Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates to mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. May function as a tumor suppressor. Self-associates. Interacts with BNIP3 and STEAP3. Interacts with SPATA18/MIEAP. Interacts with human adenovirus-2 E1B 19 kDa protein. Belongs to the NIP3 family.

Protein type: Apoptosis; Membrane protein, integral; Mitochondrial; Endoplasmic reticulum; Nuclear envelope

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; integral to membrane; nuclear envelope; intrinsic to membrane

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protein heterodimerization activity; lamin binding

Biological Process: viral reproduction; positive regulation of apoptosis; defense response to virus; negative regulation of apoptosis