Rabbit Bim Polyclonal Antibody | anti-BCL2L11 antibody

Bim Antibody

Cat. Num. AAA151226

• Gene Names: BCL2L11; BAM; BIM; BOD
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Bim Antibody is affinity chromatography purified via peptide column.
• Synonyms: Bim; Polyclonal Antibody; Bim Antibody; BAM; BIM; BOD; Bcl-2-like protein 11; Bcl2-interacting mediator of cell death; Bcl2-L-11; BCL2-like 11 (apoptosis facilitator); anti-BCL2L11 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Bim Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 198

• Applicable Applications for anti-BCL2L11 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Bim antibody can be used for detection of Bim by Western blot at 1 mug/mL. A 23 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Bim antibody was raised against a 20 amino acid peptide corresponding to amino acids near the amino terminus of human Bim.
• Buffer: Bim Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Bim antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Immunohistochemistry (IHC)

(Immunohistochemistry of Bim in human skin cancer cells with Bim antibody at 20 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of Bim in K562 cells with Bim Antibody at 20 μg/mL.)

Related Product Information for anti-BCL2L11 antibody

Bim Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat. Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.

NCBI and Uniprot Product Information

• NCBI GI #: 18202042
• NCBI GeneID: 10018
• UniProt Accession #: O43521; O43522; Q0MSE7; Q0MSE8; Q0MSE9; Q53R28; Q6JTU6; Q6T851; Q6TE14; Q6TE15; Q6TE16; A8K2W2
• Molecular Weight: 23 kDa
• NCBI Official Full Name: Bcl-2-like protein 11
• NCBI Official Synonym Full Names: BCL2-like 11 (apoptosis facilitator)
• NCBI Official Symbol: BCL2L11
• NCBI Official Synonym Symbols: BAM; BIM; BOD
• NCBI Protein Information: bcl-2-like protein 11; bcl-2 interacting protein Bim; bcl-2-related ovarian death agonist; bcl-2 interacting mediator of cell death
• UniProt Protein Name: Bcl-2-like protein 11
• Protein Family: Bcl-2-like protein
• UniProt Gene Name: BCL2L11
• UniProt Synonym Gene Names: BIM; Bcl2-L-11
• UniProt Entry Name: B2L11_HUMAN

NCBI Description

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jun 2013]

Uniprot Description

BIM: a pro-apoptotic member of the BCL-2 protein family. Interacts with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and act as an apoptotic activator. Its expression can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role in neuronal and lymphocyte apoptosis. Transgenic studies in the mouse suggested that this protein functions as an essential initiator of the apoptosis in thymocyte-negative selection. Nineteen alternatively spliced transcript variants of this gene have been reported.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 2q13

Cellular Component: microtubule; mitochondrial outer membrane; extrinsic to membrane; endomembrane system; cytosol

Molecular Function: protein binding; microtubule binding

Biological Process: nerve growth factor receptor signaling pathway; apoptosis; positive regulation of apoptosis; cell-matrix adhesion; myeloid cell homeostasis; pigmentation during development; ear development; B cell apoptosis; positive regulation of caspase activity; cellular process regulating host cell cycle in response to virus; positive regulation of apoptosis by virus; B cell homeostasis; mammary gland development; positive regulation of neuron apoptosis; T cell homeostasis; kidney development; post-embryonic organ morphogenesis; spleen development; caspase activation; thymus development; positive regulation of protein homooligomerization; in utero embryonic development; male gonad development; positive regulation of cell cycle; lumen formation; regulation of organ growth; odontogenesis of dentine-containing teeth; DNA damage response, signal transduction resulting in induction of apoptosis; spermatogenesis; regulation of pigmentation during development; brain development