Rabbit Bim Polyclonal Antibody | anti-Bim antibody

Bim Antibody

Cat. Num. AAA9400935

• Gene Names: BCL2L11; BAM; BIM; BOD
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: Bim; Polyclonal Antibody; Bim Antibody; Bod; anti-Bim antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 198

• Applicable Applications for anti-Bim antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a peptide corresponding to amino acids near the center of human Bim. The sequence is identical to that of mouse and rat.
• Target Name: Bim
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Bim in K562 cell lysates with Bim antibody (IN2) at (A) 2.5 and (B) 5 ug/mL.)

Immunocytochemistry (ICC)

(Immunocyochemistry of BIM in K562 cells with Bim antibody at 10 ug/mL.)

Related Product Information for anti-Bim antibody

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat. Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.

Product Categories/Family for anti-Bim antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 20336315
• NCBI GeneID: 10018
• NCBI Accession #: NP_619527
• NCBI GenBank Nucleotide #: NM_138621.4
• Molecular Weight: 8,523 Da
• NCBI Official Full Name: bcl-2-like protein 11 isoform 1
• NCBI Official Synonym Full Names: BCL2-like 11 (apoptosis facilitator)
• NCBI Official Symbol: BCL2L11
• NCBI Official Synonym Symbols: BAM; BIM; BOD
• NCBI Protein Information: bcl-2-like protein 11
• UniProt Protein Name: Bcl-2-like protein 11
• UniProt Gene Name: BCL2L11
• UniProt Synonym Gene Names: BIM; Bcl2-L-11
• UniProt Entry Name: B2L11_HUMAN

NCBI Description

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jun 2013]

Uniprot Description

BIM: a pro-apoptotic member of the BCL-2 protein family. Interacts with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and act as an apoptotic activator. Its expression can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role in neuronal and lymphocyte apoptosis. Transgenic studies in the mouse suggested that this protein functions as an essential initiator of the apoptosis in thymocyte-negative selection. Nineteen alternatively spliced transcript variants of this gene have been reported.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 2q13

Cellular Component: microtubule; mitochondrial outer membrane; extrinsic to membrane; endomembrane system; cytosol

Molecular Function: protein binding; microtubule binding

Biological Process: nerve growth factor receptor signaling pathway; apoptosis; positive regulation of apoptosis; cell-matrix adhesion; myeloid cell homeostasis; pigmentation during development; ear development; B cell apoptosis; positive regulation of caspase activity; cellular process regulating host cell cycle in response to virus; positive regulation of apoptosis by virus; mammary gland development; B cell homeostasis; positive regulation of neuron apoptosis; T cell homeostasis; kidney development; post-embryonic organ morphogenesis; spleen development; caspase activation; thymus development; in utero embryonic development; positive regulation of protein homooligomerization; male gonad development; positive regulation of cell cycle; lumen formation; regulation of organ growth; odontogenesis of dentine-containing teeth; DNA damage response, signal transduction resulting in induction of apoptosis; spermatogenesis; regulation of pigmentation during development; brain development