Rabbit anti-Human Bid Polyclonal Antibody | anti-Bid antibody

Bid, Phosphorylated (Ser65) (BH3-interacting Domain Death Agonist, p22 BID) (Azide free) (HRP)

Cat. Num. AAA6465322

• Gene Names: BID; FP497
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Bid; Polyclonal Antibody; Phosphorylated (Ser65) (BH3-interacting Domain Death Agonist; p22 BID) (Azide free) (HRP); anti-Bid antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Bid when phosphorylated at Ser65.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-Bid antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser65 of human Bid, conjugated to KLH.
• Conjugate: HRP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-Phospho-Bid-S65 Pab is used in Western blot to detect Phospho-Bid-S65 in Jurkat tissue lysate)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-Bid antibody

Antibodies; Apoptosis

References

1. Liu, J., et al., Biochem. Biophys. Res. Commun. 330(3): 865-870 (2005). 2. Broaddus, V.C., et al., J. Biol. Chem. 280(13): 12, 486-12, 493 (2005). 3. Weng, C., et al., J. Biol. Chem. 280(11): 10, 491-10, 500 (2005). 4. Gong, X.M., et al., J. Biol. Chem. 279(28): 28, 954-28, 960 (2004). 5. Garcia-Saez, A.J., et al., Biochemistry 43(34): 10, 930-10, 943 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 37574726
• NCBI GeneID: 637
• NCBI Accession #: NP_932070
• NCBI GenBank Nucleotide #: NM_197966
• UniProt Accession #: P55957
• Molecular Weight: 27kDa
• NCBI Official Full Name: BH3-interacting domain death agonist isoform 1
• NCBI Official Synonym Full Names: BH3 interacting domain death agonist
• NCBI Official Symbol: BID
• NCBI Official Synonym Symbols: FP497
• NCBI Protein Information: BH3-interacting domain death agonist
• UniProt Protein Name: BH3-interacting domain death agonist
• Protein Family: Bidirectional sugar transporter
• UniProt Gene Name: BID
• UniProt Synonym Gene Names: BID
• UniProt Entry Name: BID_HUMAN

NCBI Description

This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]

Uniprot Description

BID: a pro-apoptotic member of the Bcl-2 superfamily. Targets intracellular membranes and contains a BH3 death domain. Heterodimerizes with either the pro-apoptotic protein BAX or the anti-apoptotic protein BCL2, antagonizing its protective effect. The activity of BID is regulated by Caspase 8-mediated cleavage, exposing the BH3 domain and significantly changing the surface charge and hydrophobicity, causing translocation to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced variants have been found.

Protein type: Apoptosis; Mitochondrial

Chromosomal Location of Human Ortholog: 22q11.1

Cellular Component: mitochondrial outer membrane; membrane; mitochondrion; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding; death receptor binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of apoptosis; apoptosis; positive regulation of protein homooligomerization; DNA damage response, signal transduction; response to estradiol stimulus; regulation of cell proliferation; positive regulation of protein oligomerization; neuron apoptosis; apoptotic mitochondrial changes; induction of apoptosis via death domain receptors; brain development; protein targeting to mitochondrion; protein homooligomerization