Western Blot (WB)
(Western blot analysis of extracts from K562 cells, treated with insulin (0.01U/ml, 15mins), using p57 Kip2 (Phospho-Thr310) antibody.)
anti-Human, Mouse p57 Kip2 Antibody | anti-p57 Kip2 antibody
p57 Kip2 (Phospho-Thr310) Antibody
Gene Names
CDKN1C; BWS; WBS; p57; BWCR; KIP2; p57Kip2
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
p57 Kip2; p57 Kip2 (Phospho-Thr310) Antibody; CDKN1C; CDN1C; CDNC; Cyclin-dependent kinase inhibitor 1C; Cyclin-dependent kinase inhibitor p57; KIP2; anti-p57 Kip2 antibody
Reactivity
Human, Mouse
Specificity
p57 Kip2 (Phospho-Thr310) antibody detects endogenous levels of p57 Kip2 only when phosphorylated at threonine 310.
Purity/Purification
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
316
Applicable Applications for anti-p57 Kip2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:1000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human p57 Kip2 around the phosphorylation site of threonine 310 (E-Q-TP-P-R).
All Sites
Human: Thr310
Mouse: Thr342
Mouse: Thr342
Preparation and Storage
Store at-20 degree C for 1 year.
Western Blot (WB)
(Western blot analysis of extracts from K562 cells, treated with insulin (0.01U/ml, 15mins), using p57 Kip2 (Phospho-Thr310) antibody.)
Western Blot (WB)
(Western blot analysis of extracts from K562 cells, treated with insulin (0.01U/ml, 15mins), using p57 Kip2 (Phospho-Thr310) antibody.)
Product Categories/Family for anti-p57 Kip2 antibody
References
Matsuoka S., Genes Dev. 9:650-662(1995). Reid L.H., Cancer Res. 56:1214-1218(1996). Tokino T., Hum. Genet. 97:625-631(1996). Lam W.W.K., J. Med. Genet. 36:518-523(1999).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31,040 Da
NCBI Official Full Name
cyclin-dependent kinase inhibitor 1C isoform a
NCBI Official Synonym Full Names
cyclin dependent kinase inhibitor 1C
NCBI Official Symbol
CDKN1C
NCBI Official Synonym Symbols
BWS; WBS; p57; BWCR; KIP2; p57Kip2
NCBI Protein Information
cyclin-dependent kinase inhibitor 1C
UniProt Protein Name
Cyclin-dependent kinase inhibitor 1C
UniProt Gene Name
CDKN1C
UniProt Synonym Gene Names
KIP2
NCBI Description
This gene is imprinted, with preferential expression of the maternal allele. The encoded protein is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]
Uniprot Description
Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life.MiscellaneousPaternally imprinted, therefore most expression comes from the maternal allele.
Research Articles on p57 Kip2
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Product Notes
The p57 Kip2 cdkn1c (Catalog #AAA858913) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The p57 Kip2 (Phospho-Thr310) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's p57 Kip2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000. Researchers should empirically determine the suitability of the p57 Kip2 cdkn1c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p57 Kip2, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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