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Immunofluorescence (IF) (Immunofluorescence analysis of methanol-fixed A431, using BHMT antibody at 1:500 dilution.)

Rabbit BHMT Polyclonal Antibody | anti-BHMT antibody

BHMT Antibody

Gene Names
BHMT; BHMT1; HEL-S-61p
Applications
Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
BHMT; Polyclonal Antibody; BHMT Antibody; Affinity Purified Rabbit Polyclonal BHMT Antibody; Polyclonal BHMT antibody; Anti-BHMT antibody; betaine--homocysteine S-methyltransferase antibody; betaine--homocysteine S-methyltransferase 1 antibody; BHMT1 antibody; BHMT antibody; anti-BHMT antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
406
Applicable Applications for anti-BHMT antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:3000
Immunogen
BHMT antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 1 and 243 of BHMT
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence analysis of methanol-fixed A431, using BHMT antibody at 1:500 dilution.)

Immunofluorescence (IF) (Immunofluorescence analysis of methanol-fixed A431, using BHMT antibody at 1:500 dilution.)

Western Blot (WB)

(Western blot analysis of 30 ug whole cell lysate (A:Hep G2) using a 10% SDS PAGE gel and BHMT antibody at a dilution of 1:1000)

Western Blot (WB) (Western blot analysis of 30 ug whole cell lysate (A:Hep G2) using a 10% SDS PAGE gel and BHMT antibody at a dilution of 1:1000)
Related Product Information for anti-BHMT antibody
This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.
Product Categories/Family for anti-BHMT antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
635
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
45 kDa
NCBI Official Full Name
betaine--homocysteine S-methyltransferase 1
NCBI Official Synonym Full Names
betaine--homocysteine S-methyltransferase
NCBI Official Symbol
BHMT
NCBI Official Synonym Symbols
BHMT1; HEL-S-61p
NCBI Protein Information
betaine--homocysteine S-methyltransferase 1
UniProt Protein Name
Betaine--homocysteine S-methyltransferase 1
UniProt Gene Name
BHMT
UniProt Entry Name
BHMT1_HUMAN

NCBI Description

This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

BHMT: Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.

Protein type: Amino Acid Metabolism - glycine, serine and threonine; Amino Acid Metabolism - cysteine and methionine; EC 2.1.1.5; Methyltransferase

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: protein complex; cytoplasm; cytosol

Molecular Function: betaine-homocysteine S-methyltransferase activity; homocysteine S-methyltransferase activity; zinc ion binding; protein complex binding

Biological Process: S-methylmethionine cycle; protein amino acid methylation; betaine catabolic process; sulfur amino acid metabolic process; S-adenosylmethionine metabolic process; response to organic nitrogen; regulation of homocysteine metabolic process; betaine metabolic process

Research Articles on BHMT

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Product Notes

The BHMT bhmt (Catalog #AAA5311883) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's BHMT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:3000. Researchers should empirically determine the suitability of the BHMT bhmt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BHMT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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