Rabbit anti-Human BFAR Polyclonal Antibody | anti-BFAR antibody

BFAR (Bifunctional Apoptosis Regulator, RING Finger Protein 47, BAR, RNF47)

Cat. Num. AAA6003120

• Gene Names: BFAR; BAR; RNF47
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Serum; Serum
• Synonyms: BFAR; Polyclonal Antibody; BFAR (Bifunctional Apoptosis Regulator; RING Finger Protein 47; BAR; RNF47); Anti -BFAR (Bifunctional Apoptosis Regulator; anti-BFAR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BFAR.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid, 0.05% sodium azide.

• Applicable Applications for anti-BFAR antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.; Dilution: Western Blot: 1:1000-1:2000; Immunohistochemistry (formalin fixed paraffin embedded): 1:1000-1:5000; Immunoprecipitation: 1:50-1:200; Immunohistochemistry: Frozen
• Immunogen: Synthetic peptide corresponding to aa3-21 (EPQKSYVNTMDLERDEPLK) of human BAR; GenBank no. NP_057645.1.
• Positive Control: CNS, neuronal cell lines
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of BAR in normal prostate and prostate carcinoma tissue lysates. 25ug protein was loaded per lane. Tissue lysates from 15 different prostate carcinoma patients show variable expression of BAR with respect to banding patterns and amount of BAR expression. Major BAR bands typically migrate as a single band or as a doublet. These bands are typically observed at ~50-54kD. This is higher than the predicted molecular weight from the 450aa BAR sequence, and may represent phosphorylation or other post-translational modifications. N = tissue lysate from normal prostate.)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human prostate carcinoma tissue array stained for BAR expression using MBS6003120 at 1:2000. Hematoxylin-eosin counterstain. Variable BAR expression is seen between patient samples.)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded tissue sections stained for BAR expression using MBS6003120 at 1:2000. A. Two cores from a human gliobastoma tissue microarray: 1 = fibrillary astrocytoma (grade I), and 2 = anaplastic glioma (grade III). B. Higher magnification from the fibrillary astrocytoma (shown in A). C. Higher magnification from the anaplastic glioma (shown in A). D. Normal human brain striatum with positive medium spiny neurons, the major neuronal cell type of the striatum. Hematoxylin-eosin counterstain.)

Related Product Information for anti-BFAR antibody

BAR (bifunctional apoptosis regulator) is a multidomain protein that was originally identified as an inhibitor of Bax-induced apoptosis (Zhang et al, 2000). Apoptosis induction can be divided up into two major pathways, extrinsic and intrinsic. The extrinsic pathway is represented by death receptor signaling and the intrinsic pathway depends on mitochondrial events. BAR is in anchored in intracellular membranes and is thought to be a scaffold protein that may bridge components of both extrinsic and intrinsic apoptosis pathways through its antiapoptotic domains: 1. BAR contains a DED (death effector domain)-like protein interaction domain that suppresses death receptor apoptosis signaling pathways. Death receptors such as the TNF (tumor necrosis factor)-family contain protein interaction domains called DD (death domains) in their cytosolic regions. DD-containing TNF receptor family members such as Fas aggregate upon binding ligand and bind to an adaptor protein FADD which contains both DD and DED domains. The Fas/FADD complexes bind to the caspase family members such as 8 and 10 which contain DEDs in their N-terminal prodomain. This is followed by proteolytic processing and caspase activation, thereby initiating a signal transduction cascade leading to activation of downstream effector caspases, substrate cleavage, andultimate cell death. DED-containing antiapoptotic proteins like BAR function as transdominant apoptosis inhibitors by competing for binding to the DED domains of proapoptotic proteins like Fadd, caspase-8 and caspase-10, thereby preventing assembly of functional death-inducing complexes and hence activation of downstream apoptosis signaling cascades. 2. BAR also contains a domain that mediates interactions with Bcl-2 family proteins and that is required for suppression of Bax-induced cell death in yeast and mammalian cells. Although the physiological functions of BAR remain to be elucidated. BAR is highly expressed in the brain and expression patterns as well as functionalDta with neuronal cell lines suggest that BAR is involved in regulating neuronal survival (Roth et al. 2003). Additionally, subcellular localization studies indicate that BAR predominantly localizes to the endoplasmic reticulum (ER), irrespective of cell type. Bcl-2 family proteins also localize to the ER. There is important crosstalk between the ER and mitochondria in the execution of cell death. It is thought that both BAR and Bcl-2 proteins play a role in regulating cell death/apoptosis induced by ER stress. Dysregulation of ER homeostasis and apoptosis is thought to be involved in the pathogenesis of some human neuronal diseases, including Alzheimer's, Parkinson's, polyglutamine diseases, nueronal storage diseases, prion dieases, as well as acute neurodegeration from brain trauma (reviewed in Lindholm et al, 2006). Since BAR is normally widely expressed in the brain, it may have a cytoprotective function in helping neurons to survive for the entire lifetime of the organism by playing a central role in inhibiting ER initiated apoptosis.

Product Categories/Family for anti-BFAR antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 7706091
• NCBI GeneID: 51283
• NCBI Accession #: NP_057645.1
• NCBI GenBank Nucleotide #: NM_016561.2
• UniProt Accession #: Q9NZS9; A8K4Z9; D3DUG8
• Molecular Weight: 52,738 Da
• NCBI Official Full Name: bifunctional apoptosis regulator
• NCBI Official Synonym Full Names: bifunctional apoptosis regulator
• NCBI Official Symbol: BFAR
• NCBI Official Synonym Symbols: BAR; RNF47
• NCBI Protein Information: bifunctional apoptosis regulator; RING finger protein 47; bifunctional apoptosis inhibitor
• UniProt Protein Name: Bifunctional apoptosis regulator
• Protein Family: Bifunctional apoptosis regulator
• UniProt Gene Name: BFAR
• UniProt Synonym Gene Names: BAR; RNF47
• UniProt Entry Name: BFAR_HUMAN

Uniprot Description

BFAR: Apoptosis regulator. Has anti-apoptotic activity, both for apoptosis triggered via death-receptors and via mitochondrial factors.

Protein type: Apoptosis; Ubiquitin conjugating system; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p13.12

Cellular Component: endoplasmic reticulum membrane; membrane; integral to plasma membrane

Molecular Function: zinc ion binding; structural molecule activity

Biological Process: apoptosis; negative regulation of apoptosis