Rabbit beta Tubulin Polyclonal Antibody | anti-Tubb5 antibody

beta Tubulin Antibody

Cat. Num. AAA9231279

• Gene Names: Tubb5; AA408537; AI596182; M(beta)5; B130022C14Rik
• Reactivity: Mouse; Predicted: C.Elegans, Chicken, Drosophila, Human, Monkey, Rat, Bovine, Xenopus, Hamster, Pig
• Applications: Western Blot, ELISA
• Purity: Purified through a protein A column, followed by peptide affinity purification.
• Synonyms: beta Tubulin; Polyclonal Antibody; beta Tubulin Antibody; Purified Rabbit Polyclonal Antibody (Pab); Tubulin beta-5 chain; Tubb5; anti-Tubb5 antibody

• Host: Rabbit
• Reactivity: Mouse; Predicted: C.Elegans, Chicken, Drosophila, Human, Monkey, Rat, Bovine, Xenopus, Hamster, Pig
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This beta Tubulin antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 298-328 amino acids from the mouse region of mouse beta Tubulin.
• Purity/Purification: Purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 444

• Applicable Applications for anti-Tubb5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:2000
• Antigen Type: Synthesized Peptide
• Antigen Source: Human
• Tissue Location: Ubiquitously expressed with highest levels in spleen, thymus and immature brain. Expressed in embryonic brain, including throughout the developing cortex and in the subventricular zone. Also found in radial glial cells, intermediate progenitors, migrating neurons and postmitotic neurons (PubMed:23246003). Expressed in skin and developing hair follicle (PubMed:26637975).
• Cellular Location: Cytoplasm, cytoskeleton
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks.; For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-beta Tubulin Antibody at 1:2000 dilution + Mouse brain lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Anti-beta Tubulin Antibody at 1:2000 dilution + Mouse brain lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-Tubb5 antibody

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Product Categories/Family for anti-Tubb5 antibody

Primary Antibodies; Signal Transduction

References

Wang D.,et al.J. Cell Biol. 103:1903-1910(1986). Carninci P.,et al.Science 309:1559-1563(2005). Church D.M.,et al.PLoS Biol. 7:E1000112-E1000112(2009). Lubec G.,et al. Submitted (JUL-2007) to UniProtKB.Lewis S.A.,et al.J. Cell Biol. 101:852-861(1985).

NCBI and Uniprot Product Information

• NCBI GI #: 7106439
• NCBI GeneID: 22154
• NCBI Accession #: NP_035785.1
• NCBI GenBank Nucleotide #: NM_011655.5
• UniProt Accession #: P99024; P05218; Q3TFB6; Q3THH9; Q3TIL1; Q3UAV4; Q3UF52; Q8WUC1; Q9CY33; B1B178
• Molecular Weight: 49671
• NCBI Official Full Name: tubulin beta-5 chain
• NCBI Official Synonym Full Names: tubulin, beta 5 class I
• NCBI Official Symbol: Tubb5
• NCBI Official Synonym Symbols: AA408537; AI596182; M(beta)5; B130022C14Rik
• NCBI Protein Information: tubulin beta-5 chain
• UniProt Protein Name: Tubulin beta-5 chain
• Protein Family: Tubulin
• UniProt Gene Name: Tubb5

Uniprot Description

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.