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Western Blot (WB) (Western blot detection of Tubulin beta in COS7,LNCap,MCF7 and C2C12 cell lysates and using Tubulin beta mouse mAb (1:5000 diluted).Predicted band size: 55KDa.Observed band size: 55KDa.)

Mouse beta Tubulin Monoclonal Antibody | anti-TUBB antibody

beta Tubulin Antibody

Gene Names
TUBB; M40; TUBB1; TUBB5; CDCBM6; OK/SW-cl.56
Reactivity
Human, Mouse, Goat
Applications
Western Blot, Immunocytochemistry
Synonyms
beta Tubulin; Monoclonal Antibody; beta Tubulin Antibody; Purified Mouse Monoclonal Antibody (Mab); beta 4 tubulin; Beta 5 tubulin; beta Ib tubulin; M40; TUBB1; TUBB5; tubulin beta 1 chain; Tubulin beta 4 chain; tubulin beta 5 chain; Tubulin beta; Tubulin beta chain; tubulin beta polypeptide; anti-TUBB antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse, Goat
Clonality
Monoclonal
Form/Format
Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide,0.1mg/mlBSA and 50% glycerol.
Sequence Length
464
Applicable Applications for anti-TUBB antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
WB ~~ 1:5000
ICC ~~ 1:500
Antigen Source
Human
Antigen Type
Recombinant Protein
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot detection of Tubulin beta in COS7,LNCap,MCF7 and C2C12 cell lysates and using Tubulin beta mouse mAb (1:5000 diluted).Predicted band size: 55KDa.Observed band size: 55KDa.)

Western Blot (WB) (Western blot detection of Tubulin beta in COS7,LNCap,MCF7 and C2C12 cell lysates and using Tubulin beta mouse mAb (1:5000 diluted).Predicted band size: 55KDa.Observed band size: 55KDa.)

Immunocytochemistry (ICC)

(Immunocytochemistry staining of HeLa cells fixed with 4% paraformaldehyde and using anti-Tubulin beta mouse mAb (dilution 1:500).)

Immunocytochemistry (ICC) (Immunocytochemistry staining of HeLa cells fixed with 4% paraformaldehyde and using anti-Tubulin beta mouse mAb (dilution 1:500).)
Related Product Information for anti-TUBB antibody
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Product Categories/Family for anti-TUBB antibody
References
Lee M.G.-S.,et al.Cell 33:477-487(1983). Hall J.L.,et al.Mol. Cell. Biol. 3:854-862(1983). Crabtree D.V.,et al.Bioorg. Med. Chem. 9:1967-1976(2001). Yu W.,et al.Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases. Shiina S.,et al.Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49671
NCBI Official Full Name
tubulin beta chain isoform a
NCBI Official Synonym Full Names
tubulin, beta class I
NCBI Official Symbol
TUBB
NCBI Official Synonym Symbols
M40; TUBB1; TUBB5; CDCBM6; OK/SW-cl.56
NCBI Protein Information
tubulin beta chain
UniProt Protein Name
Tubulin beta chain
Protein Family
UniProt Gene Name
TUBB
UniProt Synonym Gene Names
TUBB5
UniProt Entry Name
TBB5_HUMAN

NCBI Description

This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014]

Uniprot Description

TUBB: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. May interact with RNABP10. Interacts with PIFO/C1orf88. Ubiquitously expressed with highest levels in spleen, thymus and immature brain. Belongs to the tubulin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 6p21.33

Cellular Component: microtubule; cytoskeleton; nuclear envelope lumen; nucleus; cytosol

Molecular Function: GTPase activity; protein domain specific binding; protein binding; GTP binding; structural constituent of cytoskeleton; MHC class I protein binding; ubiquitin protein ligase binding; protein complex binding; GTPase activating protein binding; structural molecule activity

Biological Process: protein polymerization; cell division; natural killer cell mediated cytotoxicity; metabolic process; organelle organization and biogenesis; mitotic cell cycle; cytoskeleton-dependent intracellular transport; spindle assembly; cell motility; G2/M transition of mitotic cell cycle; cellular process; microtubule-based process

Disease: Cortical Dysplasia, Complex, With Other Brain Malformations 6

Research Articles on TUBB

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Product Notes

The TUBB tubb (Catalog #AAA9215404) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The beta Tubulin Antibody reacts with Human, Mouse, Goat and may cross-react with other species as described in the data sheet. AAA Biotech's beta Tubulin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). WB ~~ 1:5000 ICC ~~ 1:500. Researchers should empirically determine the suitability of the TUBB tubb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta Tubulin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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