Goat Tissue factor pathway inhibitor ELISA Kit | TFPI elisa kit

Goat Tissue factor pathway inhibitor ELISA Kit

Cat. Num. AAA735288

• Gene Names: TFPI; EPI; TFI; LACI; TFPI1
• Reactivity: Goat
• Synonyms: Tissue factor pathway inhibitor; Goat Tissue factor pathway inhibitor ELISA Kit; TFPI elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This TFPI ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat TFPI. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TFPI elisa kit

Principle of the assay: TFPI ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TFPI antibody and an TFPI-HRP conjugate. The assay sample and buffer are incubated together with TFPI-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TFPI concentration since TFPI from samples and TFPI-HRP conjugate compete for the anti-TFPI antibody binding site. Since the number of sites is limited, as more sites are occupied by TFPI from the sample, fewer sites are left to bind TFPI-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TFPI concentration in each sample is interpolated from this standard curve.

Product Categories/Family for TFPI elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 73760409
• NCBI GeneID: 7035
• NCBI Accession #: NP_001027452.1
• NCBI GenBank Nucleotide #: NM_001032281.2
• UniProt Accession #: P10646; O95103; Q53TS4; Q59EE5
• Molecular Weight: 35,015 Da
• NCBI Official Full Name: tissue factor pathway inhibitor isoform b
• NCBI Official Synonym Full Names: tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
• NCBI Official Symbol: TFPI
• NCBI Official Synonym Symbols: EPI; TFI; LACI; TFPI1
• NCBI Protein Information: tissue factor pathway inhibitor; anti-convertin; extrinsic pathway inhibitor
• UniProt Protein Name: Tissue factor pathway inhibitor
• Protein Family: Tissue factor pathway inhibitor
• UniProt Gene Name: TFPI
• UniProt Synonym Gene Names: LACI; TFPI1; TFPI; EPI; LACI
• UniProt Entry Name: TFPI1_HUMAN

NCBI Description

This gene encodes a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. The encoded protein is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been confirmed. [provided by RefSeq, Jul 2008]

Uniprot Description

TFPI: Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor; Secreted; Membrane protein, GPI anchor; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2q32

Cellular Component: extracellular space; cell surface; endoplasmic reticulum; extracellular region; plasma membrane

Molecular Function: serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity

Biological Process: blood coagulation, extrinsic pathway; blood coagulation