BCL2 recombinant protein

BCL2 recombinant protein

Cat. Num. AAA515325

• Gene Names: BCL2; Bcl-2; PPP1R50
• Applications: Western Blot
• Synonyms: BCL2; BCL2 recombinant protein; Bcl-2

• Host: E Coli
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Length: 6492

• Applicable Applications for BCL2 recombinant protein: Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: GST tag
• Expression System: E.coli
• Source Note: Recombinant full-length human BCL2 was expressed in E. coli cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for BCL2 recombinant protein

Recombinant full-length human BCL2 was expressed in E. coli cells using an N-terminal GST tag.

Scientific Background: BCL2 gene encodes an integral inner mitochondrial membrane protein that acts as an antiapoptotic protein (1). The protein BAD can antagonize both the cell cycle and antiapoptotic functions of BCL2 through binding to the BH3 domain. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma (2). BCL2 is phosphorylated on specific serine/threonine residues within the unstructured loop in response to diverse stimuli and such phosphorylation has been associated with the loss of the biological function of BCL2.

Product Categories/Family for BCL2 recombinant protein

Apoptosis Proteins; Signaling Proteins - Apoptosis Proteins

References

1.Hockenbery, D. et al: Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 348: 334-336, 1990.
2.Tsujimoto, Y. et al: Involvement of the bcl-2 gene in human follicular lymphoma. Science 228: 1440-1443, 1985

NCBI and Uniprot Product Information

• NCBI GI #: 72198188
• NCBI GeneID: 596
• NCBI Accession #: NP_000624.2
• NCBI GenBank Nucleotide #: NM_000633
• UniProt Accession #: P10415; P10416; Q13842; Q16197
• Molecular Weight: ~51kDa
• NCBI Official Full Name: Homo sapiens B-cell CLL/lymphoma 2 (BCL2), transcript variant alpha, mRNA
• NCBI Official Synonym Full Names: B-cell CLL/lymphoma 2
• NCBI Official Symbol: BCL2
• NCBI Official Synonym Symbols: Bcl-2; PPP1R50
• NCBI Protein Information: apoptosis regulator Bcl-2; protein phosphatase 1, regulatory subunit 50
• UniProt Protein Name: Apoptosis regulator Bcl-2
• Protein Family: Bcl2-associated agonist of cell death
• UniProt Gene Name: BCL2
• UniProt Entry Name: BCL2_HUMAN

NCBI Description

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Ref.22

Subunit structure: Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity

By similarity. Interacts with EI24

By similarity. Also interacts with APAF1, BBC3, BCL2L1, BNIPL, MRPL41 and TP53BP2. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets. Interacts with BAG1 in an ATP-dependent manner. Interacts with RAF1 (the 'Ser-338' and 'Ser-339' phosphorylated form). Interacts (via the BH4 domain) with EGLN3; the interaction prevents the formation of the BAX-BCL2 complex and inhibits the anti-apoptotic activity of BCL2. Interacts with G0S2; this interaction also prevents the formation of the anti-apoptotic BAX-BCL2 complex. Interacts with BOP. Ref.11 Ref.13 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21 Ref.23 Ref.24 Ref.25 Ref.27

Subcellular location: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein Ref.9.

Tissue specificity: Expressed in a variety of tissues.

Domain: The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.

Post-translational modification: Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A)

By similarity. Ref.15 Ref.22Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity. Ref.12Monoubiquitinated by PARK2, leading to increase its stability.

Involvement in disease: A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.

Sequence similarities: Belongs to the Bcl-2 family.