Rabbit anti-Human, Mouse Bax, BH3 Domain Polyclonal Antibody | anti-Bax antibody

Bax, BH3 Domain (Apoptosis Regulator BAX, Bcl-2-like Protein 4, Bcl2-L-4, BCL2L4)

Cat. Num. AAA626607

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Bax; BH3 Domain; Polyclonal Antibody; BH3 Domain (Apoptosis Regulator BAX; Bcl-2-like Protein 4; Bcl2-L-4; BCL2L4); Anti -Bax; anti-Bax antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Bax. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-Bax antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the N-terminal region (BH3 domain) of human Bax (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Bax Antibody(BH3) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the Bax antibody detected the Bax protein (arrow).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with Bax Antibody (BH3 Domain Specific), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HepG2 cells using Bax Antibody (BH3 Domain Specific)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of Bax (arrow) using rabbit polyclonal Bax Antibody (BH3) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Bax gene.)

Related Product Information for anti-Bax antibody

Bax belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Bax forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of the Bax gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis.

Product Categories/Family for anti-Bax antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 975870
• NCBI GeneID: 24887
• UniProt Accession #: Q63690; Q62995; Q64383; Q9JKL3
• Molecular Weight: 21,351 Da
• NCBI Official Full Name: bax
• NCBI Official Synonym Full Names: Bcl2-associated X protein
• NCBI Official Symbol: Bax
• NCBI Protein Information: apoptosis regulator BAX
• UniProt Protein Name: Apoptosis regulator BAX
• Protein Family: Protein
• UniProt Gene Name: Bax
• UniProt Entry Name: BAX_RAT

NCBI Description

Bcl2-related gene; involved in the regulation of apoptotic cell death [RGD]

Uniprot Description

BAX: Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Isoform Sigma interacts with BCL2A1 and BCL2L1 isoform Bcl-X(L). Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells. Interacts with FAIM2/LFG2. Interacts with human cytomegalovirus/HHV-5 protein vMIA/UL37. Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro- myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T- cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. Belongs to the Bcl-2 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Tumor suppressor; Membrane protein, integral; Apoptosis

Cellular Component: mitochondrial permeability transition pore complex; endoplasmic reticulum membrane; mitochondrion; endoplasmic reticulum; cell; nuclear envelope; cytosol; pore complex; mitochondrial outer membrane; membrane; cytoplasm; mitochondrial membrane; intracellular; nucleus

Molecular Function: identical protein binding; BH domain binding; protein binding; protein homodimerization activity; channel activity; protein heterodimerization activity; chaperone binding; heat shock protein binding; protein complex binding; BH3 domain binding; lipid binding

Biological Process: hypothalamus development; regulation of cell cycle; positive regulation of apoptosis; response to toxin; myeloid cell homeostasis; germ cell programmed cell death; homeostasis of number of cells; B cell apoptosis; post-embryonic development; germ cell development; regulation of mammary gland epithelial cell proliferation; spermatid differentiation; development of secondary sexual characteristics; regulation of mitochondrial membrane potential; protein insertion into mitochondrial membrane during induction of apoptosis; regulation of neuron apoptosis; establishment and/or maintenance of transmembrane electrochemical gradient; negative regulation of neuron apoptosis; kidney development; negative regulation of protein binding; response to corticosterone stimulus; response to drug; inner mitochondrial membrane organization and biogenesis; nervous system development; release of cytochrome c from mitochondria; outer mitochondrial membrane organization and biogenesis; positive regulation of B cell apoptosis; regulation of protein homodimerization activity; cellular respiration; vagina development; protein oligomerization; fertilization; induction of apoptosis via death domain receptors; DNA damage response, signal transduction resulting in induction of apoptosis; retina development in camera-type eye; negative regulation of fibroblast proliferation; reduction of endoplasmic reticulum calcium ion concentration; glycosphingolipid metabolic process; mitochondrial fragmentation during apoptosis; cerebral cortex development; response to ionizing radiation; regulation of nitrogen utilization; post-embryonic camera-type eye morphogenesis; positive regulation of pigmentation; regulation of protein heterodimerization activity; T cell homeostatic proliferation; apoptosis; neuron migration; negative regulation of peptidyl-serine phosphorylation; positive regulation of apoptosis involved in mammary gland involution; regulation of caspase activity; release of matrix enzymes from mitochondria; response to salt stress; negative regulation of cell proliferation; positive regulation of protein oligomerization; apoptotic mitochondrial changes; B cell homeostatic proliferation; B cell homeostasis; ovarian follicle development; positive regulation of neuron apoptosis; response to wounding; response to gamma radiation; response to axon injury; B cell negative selection; protein homooligomerization; leukocyte homeostasis; caspase activation; mitochondrial fusion; transformed cell apoptosis; male gonad development; Sertoli cell proliferation; response to cocaine; limb morphogenesis; odontogenesis of dentine-containing teeth; cell proliferation; neuron apoptosis; response to copper ion; homeostasis of number of cells within a tissue; spermatogenesis; blood vessel remodeling; retinal cell programmed cell death; brain development; positive regulation of release of sequestered calcium ion into cytosol; caspase activation via cytochrome c; sex differentiation; response to DNA damage stimulus