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Western Blot (WB) (Western blot analysis of p53R2 in 3T3 cell lysate with p53R2 at 1ug/mL in (A) the absence and (B) the presence of blocking peptide.)

Rabbit p53R2 Polyclonal Antibody | anti-RRM2B antibody

p53R2, NT (p53 Ribonucleotide Reductase)

Gene Names
RRM2B; P53R2; MTDPS8A; MTDPS8B
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Synonyms
p53R2; Polyclonal Antibody; NT (p53 Ribonucleotide Reductase); Anti -p53R2; anti-RRM2B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes a 39kD band. Species Crossreactivity: Mouse and rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Applicable Applications for anti-RRM2B antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 0.5-1ug/ml
Immunogen
Raised against a synthetic peptide (GDPERPEAAGLDQDER) corresponding to amino acids 2 to 17 of human p53R2 (1).
Positive Control
A431 or 3T3 cell lysate
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of p53R2 in 3T3 cell lysate with p53R2 at 1ug/mL in (A) the absence and (B) the presence of blocking peptide.)

Western Blot (WB) (Western blot analysis of p53R2 in 3T3 cell lysate with p53R2 at 1ug/mL in (A) the absence and (B) the presence of blocking peptide.)

Immunofluorescence (IF)

(Immunofluorescence of p53R2 in Human Lung tissue with p53R2 at 20ug/mL.)

Immunofluorescence (IF) (Immunofluorescence of p53R2 in Human Lung tissue with p53R2 at 20ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of p53R2 in human lung tissue with p53R2 at 1ug/mL.)

Immunohistochemistry (IHC) (Immunohistochemistry of p53R2 in human lung tissue with p53R2 at 1ug/mL.)
Related Product Information for anti-RRM2B antibody
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including p53R2 (1), Chk2 (2), p53AIP1 (3), Noxa (4), PIDD (5), and PID/MTA2 (6), were recently discovered. p53R2 is a p53 inducible gene that contains a p53 binding sequence and encodes a subunit of the enzyme ribonucleotide reductase (1,7). p53R2 is induced by the reagents, ultraviolet and g-irradiation that cause DNA damages. The product of p53R2 gene is directly involved in the p53 checkpoint for repair of damaged DNA. The isoform of the p53 family member p73 also induces p53R2 expression (8). p53R2 is an important target of p53 for tumor suppression.
Product Categories/Family for anti-RRM2B antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
40,737 Da
NCBI Official Full Name
p53-inducible ribonucleotide reductase small subunit 2 short form gamma
NCBI Official Synonym Full Names
ribonucleotide reductase M2 B (TP53 inducible)
NCBI Official Symbol
RRM2B
NCBI Official Synonym Symbols
P53R2; MTDPS8A; MTDPS8B
NCBI Protein Information
ribonucleoside-diphosphate reductase subunit M2 B; TP53-inducible ribonucleotide reductase M2 B; p53-inducible ribonucleotide reductase small subunit 2 homolog; p53-inducible ribonucleotide reductase small subunit 2-like protein; p53-inducible ribonucleotide reductase small subunit 2 short form beta
UniProt Protein Name
Ribonucleoside-diphosphate reductase subunit M2 B
UniProt Gene Name
RRM2B
UniProt Synonym Gene Names
P53R2; p53R2
UniProt Entry Name
RIR2B_HUMAN

NCBI Description

This gene encodes the small subunit of a p53-inducible ribonucleotide reductase. This heterotetrameric enzyme catalyzes the conversion of ribonucleoside diphosphates to deoxyribonucleoside diphosphates. The product of this reaction is necessary for DNA synthesis. Mutations in this gene have been associated with autosomal recessive mitochondrial DNA depletion syndrome, autosomal dominant progressive external ophthalmoplegia-5, and mitochondrial neurogastrointestinal encephalopathy. Alternatively spliced transcript variants have been described.[provided by RefSeq, Feb 2010]

Uniprot Description

RRM2B: Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage. Defects in RRM2B are the cause of mitochondrial DNA depletion syndrome type 8A (MTDPS8A). A disorder due to mitochondrial dysfunction characterized by various combinations of neonatal hypotonia, neurological deterioration, respiratory distress, lactic acidosis, and renal tubulopathy. Defects in RRM2B are the cause of mitochondrial DNA depletion syndrome type 8B (MTDPS8B). A disease due to mitochondrial dysfunction and characterized by ophthalmoplegia, ptosis, gastrointestinal dysmotility, cachexia, peripheral neuropathy. Defects in RRM2B are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 5 (PEOA5). A disorder characterized by progressive weakness of ocular muscles and levator muscle of the upper eyelid. In a minority of cases, it is associated with skeletal myopathy, which predominantly involves axial or proximal muscles and which causes abnormal fatigability and even permanent muscle weakness. Ragged-red fibers and atrophy are found on muscle biopsy. A large proportion of chronic ophthalmoplegias are associated with other symptoms, leading to a multisystemic pattern of this disease. Additional symptoms are variable, and may include cataracts, hearing loss, sensory axonal neuropathy, ataxia, depression, hypogonadism, and parkinsonism. Belongs to the ribonucleoside diphosphate reductase small chain family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleotide Metabolism - pyrimidine; Oxidoreductase; Other Amino Acids Metabolism - glutathione; Nucleotide Metabolism - purine; EC 1.17.4.1

Chromosomal Location of Human Ortholog: 8q23.1

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: metal ion binding; ribonucleoside-diphosphate reductase activity

Biological Process: deoxyribonucleoside diphosphate metabolic process; renal system process; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside and nucleotide interconversion; deoxyribonucleotide biosynthetic process; mitochondrial DNA replication; response to oxidative stress; deoxyribonucleoside triphosphate metabolic process; DNA repair; kidney development

Disease: Mitochondrial Dna Depletion Syndrome 8a (encephalomyopathic Type With Renal Tubulopathy); Progressive External Ophthalmoplegia With Mitochondrial Dna Deletions, Autosomal Dominant, 5

Research Articles on RRM2B

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Product Notes

The RRM2B rrm2b (Catalog #AAA614149) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p53R2, NT (p53 Ribonucleotide Reductase) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's p53R2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Western Blot: 0.5-1ug/ml. Researchers should empirically determine the suitability of the RRM2B rrm2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p53R2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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