Western Blot (WB)
(Western Blot: Sample: Mouse Skeletal muscle lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat TNNT1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Rat Troponin T Type 1, Slow Skeletal (TNNT1) Polyclonal Antibody | anti-TNNT1 antibody
Polyclonal Antibody to Troponin T Type 1, Slow Skeletal (TNNT1)
MGHHHHHHSG SEF-MSDTEEQEYE EEQAEDEEAV EEEAPEEPEP VAEREEERPK PSRPVVPPLI PPKIPEGERV DFDDIHRKRM EKDLLELQTL IDVHFEQRKK EEEELIALKD RIERRRAERA EQQRFRTEKE RERQAKLAEE KMRKEEEEAK KRAEDDAKKK KVLSNMGAHF GGYLVKAEQK RGKRQTGREM KLRILSERKK PLNIDYMGED QLREKAQELS EWIHQLESEK FDLMEKLK
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Mouse Skeletal muscle lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat TNNT1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Skeletal muscle lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat TNNT1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
NCBI and Uniprot Product Information
NCBI Description
human homolog plays a role in the calcium sensitivity of contraction in skeletal muscle [RGD, Feb 2006]
Uniprot Description
TNNT1: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Defects in TNNT1 are the cause of nemaline myopathy type 5 (NEM5); also known as Amish nemaline myopathy (ANM). A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 5 is a severe and progressive form common among Old Order Amish. Affected infants display tremors with hypotonia and mild contractures of the shoulders and hips. Proximal contractures progressively weaken and a pectus carinatum deformity develops before children die of respiratory insufficiency, usually in the second year. Belongs to the troponin T family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Contractile; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 1q12
Cellular Component: troponin complex
Molecular Function: tropomyosin binding; troponin T binding
Biological Process: negative regulation of muscle contraction; regulation of muscle contraction; skeletal muscle contraction; slow-twitch skeletal muscle fiber contraction; transition between fast and slow fiber
Similar Products
Product Notes
The TNNT1 tnnt1 (Catalog #AAA2007210) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Troponin T Type 1, Slow Skeletal (TNNT1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Troponin T Type 1, Slow Skeletal (TNNT1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the TNNT1 tnnt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-MSDTEE QEYE EEQAEDEEAV EEEAPEEPEP VAEREEERPK PSRPVVPPLI PPKIPEGERV DFDDIHRKRM EKDLLELQTL IDVHFEQRKK EEEELIALKD RIERRRAERA EQQRFRTEKE RERQAKLAEE KMRKEEEEAK KRAEDDAKKK KVLSNMGAHF GGYLVKAEQK RGKRQTGREM KLRILSERKK PLNIDYMGED QLREKAQELS EWIHQLESEK FDLMEKLK. It is sometimes possible for the material contained within the vial of "Troponin T Type 1, Slow Skeletal (TNNT1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
