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Western Blot (WB) (Western blot analysis of BAHD1 Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). BAHD1 (arrow) was detected using the purified Pab.)

Rabbit BAHD1 Polyclonal Antibody | anti-BAHD1 antibody

BAHD1 Antibody (C-term)

Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
BAHD1; Polyclonal Antibody; BAHD1 Antibody (C-term); Bromo adjacent homology domain-containing 1 protein; BAH domain-containing protein 1; KIAA0945; anti-BAHD1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This BAHD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 750-777 amino acids from the C-terminal region of human BAHD1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
750-777
Sequence Length
777
Applicable Applications for anti-BAHD1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of BAHD1 Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). BAHD1 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of BAHD1 Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). BAHD1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with BAHD1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human testis tissue reacted with BAHD1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(BAHD1 Antibody (C-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (BAHD1 Antibody (C-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-BAHD1 antibody
BAHD1 is a heterochromatin protein that acts as a transcription repressor and has the ability to promote the formation of large heterochromatic domains. It may act by recruiting heterochromatin proteins such as CBX5 (HP1 alpha), HDAC5 and MBD1. It represses IGF2 expression by binding to its CpG-rich P3 promoter and recruiting heterochromatin proteins.
References
Mayya V.,et.al., Sci. Signal. 2:RA46-RA46(2009).
Dephoure N., et.al., Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
84652
NCBI Official Full Name
bromo adjacent homology domain-containing 1 protein isoform a
NCBI Official Synonym Full Names
bromo adjacent homology domain containing 1
NCBI Official Symbol
BAHD1
NCBI Protein Information
bromo adjacent homology domain-containing 1 protein
UniProt Protein Name
Bromo adjacent homology domain-containing 1 protein
Protein Family
UniProt Gene Name
BAHD1
UniProt Synonym Gene Names
KIAA0945; BAH domain-containing protein 1
UniProt Entry Name
BAHD1_HUMAN

Uniprot Description

BAHD1: Heterochromatin protein that acts as a transcription repressor and has the ability to promote the formation of large heterochromatic domains. May act by recruiting heterochromatin proteins such as CBX5 (HP1 alpha), HDAC5 and MBD1. Represses IGF2 expression by binding to its CpG-rich P3 promoter and recruiting heterochromatin proteins. At specific stages of Listeria infection, in complex with TRIM28, corepresses interferon- stimulated genes, including IL28A, IL28B and IL29. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 15q15.1

Cellular Component: nucleoplasm; chromatin silencing complex; chromatin

Molecular Function: protein binding; chromatin binding

Biological Process: transcription, DNA-dependent; heterochromatin formation; chromatin silencing; negative regulation of transcription, DNA-dependent

Research Articles on BAHD1

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Product Notes

The BAHD1 bahd1 (Catalog #AAA9203483) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 750-777. The BAHD1 Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's BAHD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the BAHD1 bahd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BAHD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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